中文摘要：

为研究中药有效成分小檗碱、绿原酸和黄芩苷的抗炎机制，通过体外培养大鼠肠黏膜微血管内皮细胞（Rat intestinal mucosa microvascular endothelial cells，RIMMVECs）建立LPS损伤模型，采用免疫细胞化学方法观察了3种中药成分对LPS损伤RIMMVECs表达ICAM-1的影响，并对染色结果进行了图像灰度分析与统计学检验。研究发现，RIMVECs正常状态下低水平表达ICAM-1，用LPS刺激后ICAM—1的表达明显升高，而中药有效成分小檗碱、绿原酸和黄芩苷能够抑制LPS诱导的RIMVECs强阳性表达ICAM-1。结果表明，小檗碱、绿原酸和黄芩苷的抗炎作用与其下调ICAM-1的表达密切相关，为阐明其抗炎机制提供了数据。

英文摘要：

To investigate their anti-inflammatory mechanism, the effects of berberine （Ber.）, chlorogenic acid （Ch. A. ） and baicalin （Bai） on the expression of intercellular adhesion molecule 1 （ICAM-1） in rat intestinal mucosa microvascular endothelial cells （RIMMVECs） challeged by lipopolysaccharide （LPS） were analyzed by immunocytochemical staining, and gray scale and statistical analysis were performed. The results showed that RIMMVECs constitutively express ICAM-1 in low level and LPS significantly increased it, while LPS-induced strong expression of ICAM-1 in RIMMVECs was suppressed by Ber, Ch. A and Bai. The results indicated that the anti-inflammatory role of Ber, Ch. A and Bai is related to their downregulating effect on LPS-mediated ICAM-1 expression, which provide data for clarifying their mechanism.