利用纤维素酶的酶解作用对菠萝皮渣多糖的提取工艺进行了研究。研究了料液比、加酶量、酶解温度和酶解时间对菠萝皮渣多糖的提取率的影响,并通过正交试验确定纤维素酶法提取菠萝皮多糖的最佳工艺。正交试验结果表明提取菠萝皮渣多糖的工艺最佳条件为酶解温度48℃、酶解时间lOOmin、加酶量0.8%。在该条件下菠萝皮渣多糖的实际提取率为26.3%。该方法与其他类似提取方法相比较具有成本低,速度快,提取率高的优点。
The extraction process of pineapple peel polysaccharides using cellulose enzyme was studied in the paper. The extraction effect of the ratio of material and solvent, enzyme amount, the enzymolysis temperature and enzymolysis time were studied. And through the orthogonal test the optimum extraction technology was determined. The optimum extraction conditions were as followings : With the ratio of material and solvent of 1 : 15, adding 0. 8% enzyme at 48~C for 100min, the polysaccharides extraction rate reached 26. 3%. Compared with other method, the method has the ad- vantages of low cost, speed and high extraction.