中文摘要：

用Sephadex G-25、DEAE-Sepharose Fast Flow和Sephadex G-200方法分离纯化家蚕头部中的乙酰胆碱酯酶（AChE），以有机磷化学农药为阳性对照，比较分析几种天然产物对AChE纯酶液与粗酶液的抑制效果。结果表明，经过一系列步骤的纯化，最后所得AChE是纯度较高的酶液，并且纯化后的AChE对抑制作用更敏感。同时实验选取的大部分天然产物提取物对纯化后的AChE都有一定的抑制作用，其中绣线菊乙醇提取物对纯酶的IC50达到了0.091×10^-3 g·mL^-1。

英文摘要：

Acetylcholinesterase （AChE） was purified from the brain tissues of silkworm by Sephadex G-25, DEAE-Sepharose Fast Flow and Sephadex G-200 chromatography. Organophosphorus pesticide was used as positive control to compare the inhibitory effectson crude and purified AChE. The result indicated that the purified AChE is pure after the purification procedures, the inhibition of purified AChE was more sensitive than that of crude AChE. In addition, extracts from natural products have higher inhibition on the purified AchE than the cruded. The IC50 of purified AChE by alcohol extract of spiraea is 0.091×10^-3 g·mL^-1.