中文摘要：

目的为树突状细胞（DC）的研究和应用奠定基础。方法以重组小鼠粒细胞-巨噬细胞集落刺激因子（GM-CSF）、白细胞介素（IL-4）和脂多糖（LPS）体外诱导小鼠骨髓细胞分化为DC，倒置显微镜动态观察细胞形态学变化，流式细胞术分析细胞表面分子，混合淋巴细胞反应检测其刺激T细胞增殖能力。结果经体外诱导培养第2—8天可见大量细胞集落形成；获得的DC具有典型树突状形态，同时DC可显著刺激同种异体混合淋巴细胞增殖。结论体外诱导培养可获得小鼠骨髓来源DC，可广泛应用于临床及实验研究。

英文摘要：

Objective To investigate the cytobiological characteristics of dendritic cell （DC） for its experimental study and clinical application. Methods Mouse bone marrow cells were induced to form DCs by recombinant mouse-granulocyte and macrophage colony-stimulus factor （GM-CSF）, interleukin-4 （IL-1） and lipopolysaccharide （LPS） in vitro. The morphological changes were observed with light inverted microscope. Cell surface molecules were identified by flow cytometry. The biological function was studied with mixed lymphocyte reaction （MLR）. Results The cultured cells displayed typical morphological characteristics of DCs,and could stimulate the proliferation of allogenic mixed lymphocyte. Conclusions In vitro induction and culture can obtain DCs, and it can be widely used in the experimental and clinical study.