中文摘要：

目的探讨缺氧／复氧对小鼠骨髓树突状细胞（DC）表型及生物学活性的影响。方法培养小鼠骨髓源性DC，分为对照组及缺氧／复氧组，对照组在正常培养条件下培养，缺氧／复氧组给予缺氧气体培养4h，然后在正常培养条件下继续培养1d。应用流式细胞仪检测DC表面CD80、CD86、MHCⅡ分子的变化，ELISA法检测DC分泌TNF-α、IFN-γ和IL-12的浓度，混合淋巴细胞培养检测T细胞增殖能力，免疫细胞化学检测DC核因子-κB（NF—κB）的表达。结果缺氧／复氧可促进DC高表达CD80、CD86、MHCⅡ分子，促进Th1型细胞因子释放、NF—κB高表达及核移位，并诱导T细胞增殖。结论缺氧／复氧可刺激DC高表达表面分子，具有明显的免疫刺激活性。

英文摘要：

Objective To explore the impact of hypoxia/reoxygenation stimulation on phenotype and immune activity of dendritic cells （DCs） cultured from routine bone marrow. Methods Mouse DCs were generated from bone marrow cells and were divided into control group and hypoxia/reoxygenation group. DC in control group was cultured at normal condition, and in hypoxia/reoxygenation group was cultured at hypoxic condition for 4 h followed by cultured at normal condition for 24 h. Flow cytometry and mixed lymphocyte reaction（MLR） was used to detect the phenotype and functional properties of DCs. ELISA was used to detect the concentration of TNF-α, IFN-γ and IL-12 in the supernatant. Immunochemistry was used to detect the concentration of NF-κB. Results Hypoxia/reoxygen stimulation increased the CD80, CD86, MHC Ⅱ in the cytomembrane of DCs and TNF-α, IFN-γ, IL-12 concentration in the supernatant. Hypoxia/reoxygen stimulation also promoted the shift of NF-κB to karyon. Conclusion Under hypoxia/reoxygen stimulation, DCs express high level of surface molecules, and possess strong immune activity.