针对水华的主要物种——铜绿微囊藻,初步测定了60种植物的浸提液对其细胞增殖的抑制率。结果表明,8种植物的浸提液对铜绿微囊藻生长的抑制率达到90%以上。其中,广玉兰、龙爪槐和黄杨叶片浸提液对铜绿微囊藻生长的抑制率分别达到97.4%、95.6%和91.2%。通过计算得到培养7d后广玉兰、龙爪槐和黄杨的50%抑制效应质量浓度(EC50)分别为0.52、1.04、5.87g/L,而90%抑制效应质量浓度(EC90)分别为3.79、6.45、9.09g/L。首次发现此3种植物浸提液中富含能抑制铜绿微囊藻细胞增殖的化学物质,可以作为抑藻化感物质的一个重要来源。根据EC50和EC90对培养时间的拟合方程判断,广玉兰和龙爪槐浸提液对铜绿微囊藻的抑制效果较黄杨浸提液更为长效,且由于其抑制效果优于农作物秸秆,具有进一步研究的价值。
The inhibitory effects of extracts from 60 plant samples on Microcystis aeruginosa were investigated by measuring the cell number of Microcystis aeruginosa during the cultivation process.8 plant extracts presented remarkable anti-algae activity,their inhibitory ratio to Microcystis aeruginosa were all above 90%.The inhibitory effects of three most effective samples,Magnolia grandiflora,Sophora japonica f.pendula and Buxus sinica,were further measured,their inhibition ratio to Microcystis aeruginosa were calculated as 97.4%,95.6% and 91.2%,the medial effective concentrations (EC50) for seven days were 0.52,1.04 and 5.87 g/L,and EC90 were 3.79,6.45 and 9.09 g/L,respectively.The fitting equations between EC50 and culture time showed that the inhibitory effects of Magnolia grandiflora and Sophora japonica f.pendula to Microcystis aeruginosa would not decrease with the elongation of incubation time.The above results revealed that the three land-floras contained very high levels of anti-algae allelochemicals,they might have a great potential to treat algae bloom and control the eutrophication of water.