中文摘要：

目的建立茅苍术悬浮细胞系，研究2种内生真菌诱导子对茅苍术悬浮细胞产挥发油的影响。方法用内生真菌诱导子对茅苍术悬浮细胞进行诱导处理后超声提取茅苍术细胞中的挥发油，利用气相色谱法测定挥发油中苍术酮、苍术醇、β-桉叶醇和苍术素的量。结果获得的茅苍术悬浮细胞生长较快，于第21天生物量达到最大，为6．95g／L。未经诱导处理的茅苍术悬浮细胞中仅检出β-桉叶醇，其在培养21d的细胞中的量达到最大，为17．469μg／g。添加2种内生真菌（分别属于小克银汉霉属和孔球孢霉属，编号为AL4和AL12）的粗诱导子对茅苍术悬浮细胞生物量及挥发油积累均有影响，其中AL4粗诱导子综合效果更好。添加AL4粗诱导子到14d的茅苍术悬浮细胞培养基中，诱导子质量浓度为每升培养基含糖20mg，诱导处理7d后收集细胞，发现其干质量比同期对照提高3．31％，且检出细胞中苍术酮、苍术醇、β-桉叶醇和苍术素的干质量分别达到14．715、28．395、38．794、8．310μg／g。其中β-桉叶醇的量是对照的2．22倍。结论添加内生真菌诱导子对茅苍术悬浮细胞的生长及挥发油积累均有促进作用。

英文摘要：

Objective To establish the suspension cell line of Atractylodes lancea and to study the effect of two endophytic fungal elicitors on its essential oil production. Methods The essential oil was extracted by using ultrasonic wave after suspension cell was treated with endophytic fungal elicitors. Then, the determination of four compounds （atractylone, hinesol, β-eudesmol, and atractylodin） was carried out by gas chromatography. Results By testing in various conditions, the suspension cell line with a rapid growth rate was established. Its highest biomass （6. 95 g/L） was obtained on day 21. β-Eudesmol was the only detection in the control suspension Ceil, and its highest content （17. 469 μg/g） was also reached on day 21. The effect of crude elicitors of two endophytic fungi （belong to Cunninghamella sp. and Gilmaniella sp. respectively, named AL4 and AL12） on the cell growth and the production of essential oil were investigated. Overall AL4 elicitor got better effect. When suspension cell of 14-day-old cultures was exposed to AL4 elicitor （carbohydrate 20 mg/L medium） for 7 d, the hiomass increased 3.31% over the control, and the four compounds （atractylone. 14. 715 μg/g, hinesol. 28. 395 μg/g, β-eudesmol： 38. 794 μg/g, and atractylodin： 8. 310 μg/g） were all detected. Among them, the content of β-eudesmol reached 2.22 times as much as the control. Conclusion The cell growth and the accumulation of essential oil of A. lancea could also be promoted by adding crude elicitors of the endophytic fungi AL4 and AL12.