中文摘要：

为研究肠炎沙门菌（Salmonella enteritidis）avrA基因功能及其在感染过程中的表达分布情况,本研究通过PCR技术克隆获得了avrA基因,并构建了肠炎沙门菌AvrA原核表达载体pCold-avrA,转入大肠杆菌BL21（DE3）中进行表达;表达蛋白经纯化后免疫小鼠获得AvrA多克隆抗体,通过Western-blot检测了抗体特异性。结果显示,成功构建了表达载体pCold-avrA,并获得了纯化的AvrA蛋白,成功制备了小鼠抗AvrA蛋白的多克隆抗体,可用于检测细菌AvrA蛋白的表达。本研究成功获得纯化的AvrA蛋白及其多克隆抗体,为进一步探讨AvrA在肠炎沙门菌感染过程中所发挥功能奠定了基础。

英文摘要：

To investigate the role of avrA gene from Salmonella enteritidis in distribution and expression,we cloned the avrA gene from Salmonella enteritidis by PCR,and the gene fragment was cloned into p Cold vector,then expressed in E.coli BL21（DE3）.The AvrA protein was purified and was used as antigen to prepare polyclonal antibodies.Western-blot was used to check the specificity of the AvrA antibodies and detect the expression of AvrA protein in Salmonella.The results showed that the expression vector p Cold-avrA was successfully constructed,and the purified AvrA protein was obtained.The AvrA polyclonal antibodies were successfully prepared and could be used to detect the expression of AvrA in Salmonalla.The research provided the foundation for studying the role of avrA in the process of Salmonella enteritidis infection.