中文摘要：

本试验旨在研究补喂瘤胃液制备物对羔羊肠道黏膜和血浆中免疫球蛋白含量的影响,探讨瘤胃液制备物对新生羔羊肠道黏膜免疫及体液免疫的影响。选取50只初生体重接近的新生羔羊为模型动物,随机分为5组,每组10只。试验组羔羊1日龄开始补喂健康成年绵羊瘤胃液制备物[瘤胃液（Ⅰ）、灭菌瘤胃液（Ⅱ）、超声波破碎瘤胃液（Ⅲ）和灭菌超声波破碎瘤胃液（Ⅳ）],对照组羔羊补喂等量生理盐水,每天1次,连续5 d。在24日龄时每组选取3只羔羊屠宰,采集肠道黏膜;在羔羊14和28日龄时颈静脉采血并分离血浆。测定肠道黏膜及血浆中免疫球蛋白含量。结果显示：1）小肠黏膜蛋白质中,各试验组免疫球蛋白A（Ig A）、分泌型免疫球蛋白A（SIg A）和免疫球蛋白G（Ig G）总量均高于对照组。其中,试验Ⅲ组Ig A总量极显著高于对照组（P〈0.01）,显著高于其他试验组（P〈0.05）;试验组SIg A和Ig G总量与对照组相比无显著差异（P〉0.05）。总体来说,免疫球蛋白含量变化趋势为回肠〉十二指肠〉空肠,试验Ⅲ组免疫球蛋白总量较对照组增加较多。2）血浆中,羔羊28日龄时免疫球蛋白含量均高于14日龄。14日龄时试验各组血浆中Ig A含量差异不显著（P〉0.05）,但28日龄时试验Ⅰ组显著高于对照组（P〈0.05）,极显著高于其他试验组（P〈0.01）;试验各组Ig G含量在14和28日龄时差异均不显著（P〉0.05）。结果表明,给新生羔羊补喂不同处理的瘤胃液制备物均能提高羔羊肠黏膜免疫能力,补喂超声波破碎瘤胃液效果最佳。

英文摘要：

This experiment aimed to study the effects of oral administration of ruminal fluid preparations on immunoglobulin contents in the intestinal mucosa and plasma of lambs,to explore the effects of ruminal fluid preparations on intestinal mucosal and humoral immunity of neonatal lambs.Fifty newborn lambs with similar birth weight as model animal were divided into 5 groups with 10 lambs per group.Lambs in trial groups were fed different preparations of ruminal fluid[ruminal fluid（Ⅰ）,autoclaved ruminal fluid（Ⅱ）,ultrasonic processing ruminal fluid（Ⅲ） and autoclaved ultrasonic processing ruminal fluid（Ⅳ）]from healthy adult sheep since 1 day of age once a day over a period of 5 days,and those in control group were given the same volume of sterile saline.Three lambs per group were slaughtered to obtain small intestinal tissues on 24 days of age;plasma was collected at 14 and 28 days of age,respectively.Immunoglobulin contents in intestinal mucosa and plasma were determined.The results showed as follows：1） in intestinal mucosal protein,the contents of immunoglobulin（IgA）,secretory immunoglobin A（SIgA） and immunoglobulin G（IgG） in all trial groups were higher than those in control group.The total content of IgA in trial group Ⅲ was significantly higher than that in control group（P〈0.01）,as well as significantly higher than that in the other trial groups（P〈0.05）;there were no significant differences of the total contents of SIgA and IgG among groups（P〉0.05）.In generally,the contents of immunoglobulins showed ileum〉duodenum〉jejunum,and compared with control group,the total contents of immunoglobulins in trial group Ⅲ were greatly increased.2） In plasma,immunoglobulin contents at 28 days of age were higher than those at 14 days of age.There was no significant difference of IgA content among groups at 14 days of age（P〉0.05）,however,at 28 days of age,the content of IgA in trial group Ⅰ was significantly increased compared with control group（P〈0.05）,