中文摘要：

采用Ⅰ型胶原酶和胰蛋白酶二步消化，经体外培养获得鸡骨骼肌卫星细胞，并通过检测肌卫星细胞特异基因的表达进行鉴定。结果表明：二步消化法适用于鸡骨骼肌卫星细胞的分离和获取，此方法分离得到的鸡骨骼肌卫星细胞表达卫星细胞特异的标志基因desmin和Pax7，并具有良好的增殖和分化能力，为鸡骨骼肌细胞增殖、分化和再生机制的研究提供技术平台。

英文摘要：

Chicken skeletal muscle satellite cells were obtained by a two-step method of collagenase-Ⅰ and trypsin digestion, followed by in vitro culture. They were further identified by specific gene expression assays. In addition, cell biological characteristics and myogenic genes expression were analyzed. Results showed that satellite cells from chicken skeletal muscle tissue expressed pax7, desmin, and possessed ability to proliferate and differentiate. The technical platform was established to study the mechanism of myogenic proliferation, differentiation and muscle regeneration.