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中文摘要：
为了调查我国南方地区牛羊感染牛无浆体的状况，用PCR检测了采自四川、重庆、云南、贵州、广西、湖南、广东等七省（区、市）共685份牛和羊的血液样品的DNA。PCR方法以16SrRNA基因为靶标，采用无浆体属通用引物EEl／EE2和牛无浆体特异性引物ABlf／ABlr对采集样品的全血基因组进行套式PCR扩增。PCR结果显示：四川省样品感染率为17．59／6；重庆市样品感染率为25．7％；云南省样品感染率为29．6％；贵州省样品感染率为59．1％；广西壮族自治区样品感染率为14．0％；湖南省样品感染率为8．2％；广东省样品感染率为23．7％。调查证实了牛无浆体在我国南方地区有广泛的分布，该调查丰富了我国牛无浆体的流行病学资料。
英文摘要：
In order to investigate the prevalence of Anaplasma boris in southern China,a total of 685 blood samples of cattle and goats were collected from southern provinces, including Sichuan, Chongqing, Yunnan,Guizhou,Guangxi, Hunan and Guangdong. All the blood DNA samples were detected by nested PCR targeting 16 S rRNA gene of A. boris using Anaplasma-specific primers EE1/EE2 and A. boris-speci- fic primers AB1 f/ABlr. Based on the PCR results, the positive rate of A. boris in Sichuan, Chongqing, Yun- nan,Guizhou,Guangxi, Hunan and Guangdong was 17. 50%,25. 7%, 29. 6%, 59. 1%, 14.0%, 8. 2% and 23.7%,respectively. The study demonstrated that A. boris was widely prevalent in southern China, and enriched A. boris epidemiological data in China.
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