以生物活性高的厌氧颗粒污泥为对象,研究了U(Ⅵ)初始浓度、pH值、颗粒污泥的投加量和SO42-浓度对颗粒污泥处理含U(Ⅵ)废水的效果。结果表明:当U(Ⅵ)初始浓度为9.6 mg/L,湿颗粒污泥投加量为2 g,温度25℃,pH值为6时,对U(Ⅵ)的去除率达98.7%。随着SO_4(2-)浓度的增加,微生物活性增强,SO_4(2-)浓度为8 mmol/L时,反应9 h,溶液中的U(Ⅵ)基本去除。通过环境扫描电镜(SEM)分析颗粒污泥微观结构,SEM表明处理含U(Ⅵ)废水后的颗粒污泥主要以1~3μm的球菌和短杆菌为主,处理较高浓度(9.6 mg/L)含U(Ⅵ)废水后,生物活性较高,细菌数量较多、表面较光滑、形态较匀称,形成团聚结构。能谱分析(EDS)表明颗粒污泥内存在铀。高通量测序技术分析颗粒污泥微生物结构显示存在较多耐铀菌种,所占比重接近20%,颗粒污泥显示出良好的耐铀性。
The influence of initial U(Ⅵ) concentration, pH value, granular sludge and SO_4~(2-) concentration by granular sludge were studied,which takes uranium resistant granular sludge with high microbial activity as the object. The results indicated that the maximum adsorption occurred at pH of 6 and the removal rate was up to 98. 7 % for initial U(Ⅵ)concentration of 9.6 mg/L and adsorbent dosage of 2 g at 25 ℃. With SO_4~(2-) concentration increased,microbial activity was enhanced,and U(Ⅵ) was removed substantially 9h later,when SO_4~(2-) concentration was 8 mmol/L. Environmental scanning electron microscope(ESEM)was applied to observe the microstructure of the granular sludge. SEM results showed that spherical bacteria treating U(Ⅵ) of initial U(Ⅵ) concentration of 9.6 mg/L,which aggregated together with many bacteria,smooth surface,fine appearance and diameter of 1~3 μm,were the dominant microorganisms in granular sludge. It showed a good resistance to uranium. EDS results showed the existence of uranium in granular sludge. High-throughput sequencing technology analysis of granular sludge microbial structure shows there are many uranium-resistant bacterial species,accounting for nearly 20%. Granular sludge shows good resistance to uranium.