培养基中不同的碳源、氮源和见机盐影响冰核细菌的生长、冰核蛋白的表达及黄原胶的产率。蔗糖、麦芽糖和山梨醇均有利于冰核蛋白的生产,而且麦芽糖和山梨醇的产胶率要明显低于其它碳源:蛋白胨和玉米浆均可以作为冰核蛋白生产的优良氮源使用,无机氮不适合作为唯一氮源使用。但适量的KNO3和蛋白胨结合使用时,可以促进菌体的生长和冰核活性的表达,但同时也促进了黄原胶的形成;适量地添加无机盐对菌体的生长和冰核活性的表达有一定的促进作用,对黄原胶的合成没有明显的抑制作用。
Different carbon source, nitrogen source and inorganic salts in medium affected the cell growth, the ice nucleation activity and the xanthan gum ratio. Maltose.sorbitol and sucrose were beneficial to the producing of ice nucleative protein, and the gum ratio of maltose and sorbitol was less than the other carbon source obviously .Peptone and corn steep liquor cottld be served as good nitrogen some.Inorganic nitrogen source was not suitable for the only nitrogen source,but if addition of optimum KNO3 in peptone,the cell growth and the ice nucleation activity could be improved distinctively,but at the same time accelerated the synthesis of xanthan gum. A proper amount of mineral compounds could promote the cell growth and the synthesis of the ice nucleative protein, but could not depress the gum ratio effectively.