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中文摘要：
基于2,3-二氨基萘（DAN）与一氧化氮（NO）在海水介质中生成荧光性2,3-萘酚三唑（NAT）来测定海水中NO。确定了荧光法测定NO的条件（λex=383nm,λem=410nm）、温度和时间对DAN捕集NO的影响,以及DAN溶液对捕集NO的影响。测得NO浓度与其荧光强度之间呈线性关系,线性范围1.4～1400nmol/L,r=0.9985,P〈0.0001;检出限1nmol/L（S/N=3）;相对标准偏差1.63%（14nmol/LNO）;不存在介质干扰。同样条件下与NaOH为介质的荧光法相比,本方法将检出限提高了1个数量级。结合吹扫-捕集检测出青岛石老人（36°05′46″N;120°29′40″E）海水中NO浓度为（0.12±0.01）nmol/L,可用于监测硝普钠在海水中释放NO的规律。
英文摘要：
The determination of nitric oxide（NO） concentration in seawater medium by fluorometric method has been investigated based on the reaction that 2,3-diaminonaphthalene（DAN） can trapp NO to form the highly fluorescent,2,3-naphthotriazole（NAT）.The optimum conditions such as fluorometric determination conditions（λex=383 nm,λem=410 nm）,the influence of temperature and equilibrium time on the DAN trapping NO,and the influence of DAN solution on the trapping have been examined.The fluorometric intensity is linear with the NO concentration in the range of 1.4-1400 nmol/L with an r of 0.9985（P〈0.0001）.The detection limit is 1 nmol/L（S/N=3）;the relative standard deviation is 1.6%（14 nmol/L NO）;and there is no interference from the medium.Compared with fluorometric determination of NO concentration in NaOH medium,the method enhances one magnitude order of the detection limit.Combining the purging-trapping operation,the NO concentration of seawater in Shilaoren of Qingdao（36°05＇46″N;120°29＇40″E） was detected to be（0.12 ± 0.01） nmol/L.The method can also be applied to supervise the self-releasing NO change of sodium nitroprusside in seawater.The lower detection limit,high accuracy and good repeatability are the advantages of this method.
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