中文摘要：

随着生物分析技术和高通量筛选技术的发展，生命科学领域的试剂用量已减小至纳升乃至皮升水平，为实现高精度的微量试剂分配，研制了基于微流体脉冲驱动控制技术的双通道微量试剂分配系统，以甘油溶液为分配试剂，研究了试剂粘度、微喷嘴出口内径、驱动频率和电压幅值对分配量的影响，在系统参量驱动电压为70V、驱动频率为4Hz、微喷嘴出口内径为100μm的条件下，按照不同比例分配Na2HPO4-KH2PO4溶液，进行混合反应实验，制备具有pH值梯度的3x3磷酸盐缓冲液微阵列，并加入pH值指示剂，检测混合后溶液的酸碱性。结果表明，所制备的pH梯度微阵列样点直径相对标准偏差（n=9）为0．8％，样点反应充分、颜色均匀且梯度变化明显。基于微流体脉冲驱动控制技术的微量试剂分配方法分配精度较高、重复性好，能够实现对不同粘度试剂的自动化、并行及微量（pL级）分配，无需独立的“反应池”即可实现多种试剂的微量配比及反应。

英文摘要：

With the development of bioanalytical technique and high throughput screening techmques, me amount of reagent has been decreased to nanoliter and even picoliter level. In order to achieve high precision of micro-reagent dispensing, a two-channel micro-reagent dispensing system based on pulse driving and controlling technology of micro-fluids was developed. Then, by taking glycerine solution as dispensing reagent, the influences of viscosities, inner diameter of the micro-nozzle, driving frequency, and voltage amplitude on the dispensing volume were researched. Under the following conditions： voltage amplitude was 70 V, driving frequency was 4 Hz, inner diameter of the micro-nozzle was 100 μm, to realize mixing-reaction, different ratios of Na2HPOasolution and KH2PO4solution was dispensed for mixing-reaction experiment. Firstly, a 3 x3 phosphate buffer arrays solution with a pH gradient was prepared, pH indicator was added to detect the acidity and alkalinity of the mixed solution. Results showed that the relative standard deviation （ n = 9） of spots diameter of the prepared pH gradient arrays was 0.8% , the spots reacted fully, and also had uniform colors and significantly changed gradients. By the micro-reagent dispensing method based on pulse driving ＆ controlling of micro-fluids technology, parallel, automated and micro volume （pieoliter） dispensing with higher dispensing precision and higher repeated accuracy could be realized, and especially, micro reaction with different ratios could be reached without separate ＂reaction pools＂.