中文摘要：

目的研究绿茶中的主要活性成分表没食子儿茶酚没食子酸酯（EGCG）对中、长波紫外线辐射诱导角质形成细胞产生IL-6和NF-κB的影响。方法用ELISA法测定角质形成细胞培养上清液中IL-6水平，免疫组化和Western Blot法检测细胞中NF-κB p65的变化。结果UVB20、30mJ／cm^2和UVA10、20J／cm^2可以显著促进角质形成细胞分泌IL-6（P〈0．05），且细胞内NF-κB的产生明显增加（P〈0.05）。0.15mmol/L和0.3mmol/L的EGCG对紫外线诱导的IL-6分泌起到了抑制作用（P〈0.05），其中0.3mmol/L的EGCG作用更为明显，对紫外线辐射诱导的角质形成细胞NF-κB产生有着显著的抑制作用（P〈0.05）。结论中、长波紫外线辐射可以促进IL-6的分泌。EGCG能够抑制NF-κB易位入核，从而抑制IL-6的分泌。

英文摘要：

Objective To investigate the effects of epigallocatechin - 3- gallate （EGCG） , the major polyphenolic constituent in green tea, on UV-induced IL-6 and NF-kappaB from human keratinocytes in vitro. Methods IL-6 protein level in cellfree supernants was measured by ELISA, NF-kappaB p65 in keratinocytes was determined by immunohistochemical analysis and Western Blot. Results IL-6 and NF-kappaB protein of keratinocytes were induced by UVB 20,30 mJ/cm^2 and UVA 10,20 J/cm^2 significantly （P 〈 0.05 ）. IL-6 and NF-kappaB production induced by UV could be inhibited by EGCG（0, 15 mmol/L and 0.3 mmoL/L） （ P 〈 0. 05 ）, Conclusion NF-kappaB in keratinocytes was activated and translocated into nucleus and hence IL-6 secretion after UV irradiation, EGCG could inhibit NF-kappaB translocation to nucleus and IL-6 secretion,