中文摘要：

目的探讨表皮生长因子受体（EGF-R）反义寡核苷酸转染体外培养的表皮角质形成细胞株HaCaT后,对紫外线诱导的表皮角质形成细胞转录因子c-jun活性的影响。方法用一种高灵敏度、高特异性的比色法测定不同剂量中波紫外线（UVB）辐射后以及EGF-R反义寡核苷酸转染后紫外线辐射的角质形成细胞c-jun活性的变化;RT-PCR方法测定EGF-R反义寡核苷酸转染后EGF-RmRNA的表达。结果10、20、30mJ/cm^2 UVB辐射角质形成细胞后均可显著增强c-jun活性（P〈0.05）,不同浓度的EGF-R反义寡核苷酸转染后对30mJ/cm^2 UVB诱导的EGF-RmRNA表达和c-jun活性均有显著抑制作用（P〈0.01）。结论脂质体介导的EGF-R反义寡核苷酸转染表皮角质形成细胞可以抑制UVB辐射诱导的表皮角质形成细胞c-jun活性,表明紫外线诱导角质形成细胞c-jun激活是通过EGF-R介导的。

英文摘要：

Objective To explore the effects of antisense epidermal growth factor receptor （EGF-R） oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes after EGF-R oligodeoxynucleotldes transfect to HaCaT in vitro. Methods c-jun DNA binding activity after ultraviolet-B （ UVB） irradiation and EGF-R oligodeoxynucleotides transfection were determined with a highly sensitive and specific colorimetric method. After EGF-R oligodeoxynucleotides transfection, the mRNA level of EGF-R was detected by reverse transcription polymerase chain reaction method. Results Compared with control groups, c-jun activity in- creased significantly in UVB （ 10, 20, 30mJ/cm^2） irradiation groups （P 〈 0.05）. EGF-R mRNA and c-jun activities induced by UVB were inhibited after the keratinocytes were transfected with EGF-R antisense oligodeoxynucleotides at 2, 4 and 8 μg/ml concentrations （ P 〈 0.01 ）. Conclusion The ultraviolet-induced c-jun activity of keratinocytes can be mediated by EGF-R and inhibited by EGF-R antlsense loigodeoxynucleotldes, which is transfected to keratinocytes and mediated by lipofectamine.