中文摘要：

CO2是主要的温室气体,利用油藏进行CO2封存,并结合原油厌氧生物降解产生的H2对其进行CH4转化,将提高原油采收率,减少封存CO2长久潜在的危害.本研究以高矿化度的青海油田油井采出液为研究对象,添加碳酸氢盐进行厌氧培养,以研究其中CO2进行CH4转化的可能性.结果显示,厌氧培养体系中检测到CH4产生.且在培养过程中,碳酸氢盐添加体系内CO2相对含量降低,甲烷相对产量升高,沥青质和芳香烃组分的相对含量降低,表明青海油田油藏中存在产甲烷菌及与产甲烷过程相关的菌群,同时CO2封存利于增强原油的流动性、提高采收率.定量PCR分析表明,碳酸氢盐的添加抑制了部分微生物的生长但古菌在整个体系生命活动中的作用并未减弱,且产甲烷古菌占古菌的相对丰度明显升高.因此,在青海油田利用油藏微生物进行CO2封存并产生新甲烷能源,同时提高原油采收率具有可能性.

英文摘要：

Carbon capture and sequestration（CCS） is nowadays regarded as a strategic approach to reduce atmospheric CO2,among which CO2storage in oil reservoir is one of the reliable technologies.However,it is yet not clear whether the stored CO2 can be reduced to CH4 via methanogenesis in oil reservoir.An investigation was thus conducted with a oil production mixture sampled from Qinghai Oilfield in which HCO3-was added to mimic CO2storage in oil reservoir.The result revealed that HCO3addition led to the detection of more relative contents of methanogenic archaea and CH4,along with degradation of aromatic hydrocarbons and asphaltenes,suggesting sequestrated CO2 in Qinghai Oilfield could be transformed to CH4,and resulting in enhanced oil recovery.