目的:探索木皮比在板蓝根质量鉴定中的作用与价值。方法:采用红细胞凝集试验法和化学特征谱法分别对不同木皮比的板蓝根及其木部和皮部的抗病毒活性与化学成分进行测定,并对木皮比与活性、化学成分的相关性进行分析。结果:木皮比为1:2和1:1药材样品与其皮部样品活性相当,强于木部,药材、皮部和木部化学指纹图谱一致性、加合性较好;木皮比为2:1药材样品与其木部样品活性相当,皮部活性不明显,药材、皮部和木部化学指纹图谱一致性、加合性较差;木皮比为1:2和1:1药材样品活性强于木皮比为2:1药材样品,前二者与后者化学指纹图谱相似度较小。结论:不同木皮比板蓝根药材质量存在差异,木皮比低于1:1的质量较优;木皮比这一简单直观的生物量参数可作为板蓝根质量鉴定的指标,也为其他根类药材的质量评价提供了参考。
Objective : Explore contribution of ratio of xylem to phloem(RXP) to evaluate the quality of Radix Isatidis. Methods : Antivirus activity and chemical compositions of xylem, phloem and Radix Isatidis of different RXP were determined by RBC agglutination test and unique ehromatogram. Meanwhile, correlation between RXP and bioactivity, components was investigated. Results:the ac- tivity of medical material of Radix Isatidis whose RXP was 1 : 2 or 1 : 1 is equal to that of phloem sample, while is stronger than that of cylem sample. There was a good consistency among the chemical figureprints of three samples (Radix Isatidis, xylem and phloem). When the RXP was 2:1 ,the medical material of Radix Isatidi and its xylem had the same activity. But the activity of phloem was not obvious. Their consistency of chemical fingerprint was bad, and the activity of Radix Isatidis which had RXP of 1 : 2 or 1 : 1 was better than that formed by xylem and phloem of 2: 1. The Radix Isatidis of RXP of 1:2 or 1:1 had less similarity of chemical figureprint with that having RXP of 2: 1. Conclusion:The quality of Radix Isatidis made up by the various RXP had significant difference. Radix Isati- dis whose RXP is less than 1 : 1 had good quality and better activity. As a characteristic parameter of biologic morpha, the RXP can be applied to identifying the quality of Radix Isatidis, and also provided a reference to evaluation of other medical material of roots.