中文摘要：

建立红细胞凝集反应过程的微量量热检测方法,并用于评价不同产地板蓝根的活性差异。采用微量量热法,检测阳性参照物（植物凝集素,PHA）及8批不同产地板蓝根对20%家兔血红细胞凝集反应过程,记录凝集反应热动力学曲线,提取反应动力学参数,计算凝集效价,对结果进行主成分分析和聚类分析,并以微量板法对结果进行验证。结果表明,板蓝根对家兔血红细胞凝集反应为放热反应,反应动力学参数如反应速率常数（k：0.039～73.6min-1）、最大反应功率（Pmax：-1140.2～988.2μW）及反应焓变（Hi：-529.9～717.9μJ）等动力学参数与板蓝根浓度（0.2～1.0g·mL-1）呈良好的线性关系（r〉0.97）;主成分分析结果显示参数Pmax（531～1335μW）及Ht（585.2～989.2μJ）能够客观表征不同产地板蓝根样品凝集活性差异,与微量板法测定结果（不同产地板蓝根凝集滴度分别为3～11）基本一致。根据效价值可区分GAP产区、一般主产区和普通散种区样品,同时凝集反应曲线具有较好的特征性和产地专属性,能为板蓝根品质评价提供更加全面的综合信息。所建立的板蓝根血红细胞凝集反应的微量量热检测方法灵敏可靠,可作为凝集反应定量在线检测手段,并为板蓝根及其他具凝集活性类中药的品质评价提供新的技术参考。

英文摘要：

In this study,microcalorimetry was adopted to establish a novel method for detecting the hemagglutination process of Radix Isatidis （Banlangen in Chinese,BLG）,and to evaluate the hemagglutination activity diversity of BLG from various habitats. The hemagglutination biothermokinetics curves of positive reagent （phytohemagglutinin,PHA） and 8 batches BLG from different regions of the hemagglutination with 20% rabbit erythrocyte were recorded by microcalorimetry,then biothermokinetics parameters were abstracted,the hemagglutination utility of samples were calculated and analyzed by principal component analysis （PCA） and cluster analysis （CA）,meanwhile the results were authenticated by micro-plate agglutination. It showed that the hemagglutination was an exothermic reaction,the reaction rate constant （k：0.039-73.6 min-1）,maximum reaction power （Pmax：-1 140.2 -988.2 μW） and reaction enthalpy （Hi：-529.9 -717.9 μJ） had good linear correlation with BLG extraction concentration （0.2-1.0 g·mL-1,r 0.97）,and PCA showed Pmax （531-1 335 μW） and Ht （585.2 -989.2 μJ） could represent the hemagglutination activity diversity of BLG samples,just confirming with the results of micro-plate agglutination （the agglutination dilution was 3 -11 respectively）.According to the hemagglutination utility,the BLG samples from Good Agriculture Practice （GAP） regions,main producing area and general regions could be clustered correctly; meanwhile,the biothermokinetics curves with perfect distinctive fingerprint and specificity could give out more information for the quality control and evaluation for BLG. In conclusion,the microcalorimetry method established for detecting the hemagglutination activity of BLG samples on rabbit erythrocyte is sensitive and reliable,and could be adopted as an effective technique in detection aggulatination precisely,quantitatively and consecutively; and provide a novel approach for examining and evaluating quality for Chinese herbal medicine with aggulatinativ