将银粗糙化的蝶翅三维结构引入到表面增强拉曼散射(surface enhanced Raman scattering,SERS)基材中,提高检测效果。采用绿带翠凤蝶蝶翅作为基板,将纳米银金属颗粒负载其上,合成了经有序粗糙化的表面增强拉曼散射基底。采用场发射扫描电镜及透射电镜分析了有序银粗糙化蝶翅SERS基底的形貌特征。并以罗丹明6G作为探针分子,可检测到浓度低至10^(-14)mol/L的标样溶液,且检测效果好于二维平板SERS基底,经计算得到绿带翠凤蝶SERS基底的增强因子为1.57×10^7。在试验的基础上,结合三维有限时域差分的计算方法(finite difference time domain,FDTD),模拟计算得到绿带翠凤蝶SERS基底的电场强度E分布,其log|E|2的值为3.85,其对激发光的反射率仅为6%。研究表明银粗糙化的蝶翅三维结构,可以使表面的电场强度有显著增强。
The wing scales of Papilio maackii butterflies was employed as the scaffold for arranging silver nano-particles to improve the activity of SERS. The obtained three-dimensional micro-structures roughened with silver nano-particles were characterized by scanning electron microscopy( SEM) and transmission electron microscopy( TEM). Using Rhodamine 6G( R6G) as probe molecule,the analytical enhancement factor of Papilio maackii SERS substrate was found to reach 1. 57 × 10^7. FDTD calculation was conducted. The value of the log | E |2of Papilio maackii substrate reached up to3. 85 and the reflection was 6%. The result showed that the activity of SERS could be enhanced dramatically through arranging silver nano-particles on the micro-structure of Papilio maackii butterfly scales.