中文摘要：

目的：探讨白藜芦醇（resveratrol,Res）对视网膜色素上皮细胞增殖的影响,并初步探讨其作用机制。方法：用0、50、100、150、200和300μmol/L Res作用于ARPE-19细胞24、48和72 h后,CCK-8法检测Res对ARPE-19细胞增殖的影响,0、100、150和200μmol/L Res作用ARPE-19细胞48 h,PI单染流式细胞术检测细胞周期,Annexin V-FITC/PI双染流式细胞术检测细胞凋亡,免疫荧光化学法检测PCNA蛋白表达,实时荧光定量PCR（real-time PCR）法检测PCNA、P21和P27的mRNA表达水平。结果：CCK-8结果显示,Res抑制ARPE-19细胞增殖,呈时间和剂量依赖性;流式细胞术显示,Res使S期细胞百分比显著上升且各组凋亡率没有区别;免疫荧光显示,Res抑制PCNA蛋白表达,荧光减弱;real-time PCR显示,Res浓度依赖性地抑制PCNA的mRNA表达,而诱导表达P21和P27的mRNA。结论：Res能抑制ARPE-19细胞的增殖,使细胞阻滞在S期,其机制可能与白藜芦醇诱导P21与P27的mRNA表达、抑制PCNA的mRNA表达有关。

英文摘要：

AIM： To investigate the effects of resveratrol（ Res） on the proliferation of ARPE-19 cells and to explore the possible mechanisms. METHODS： After ARPE-19 cells were treated with Res at concentrations of 0,50,100,150,200 and 300 μmol /L for 24 h,48 h and 72 h,the effects of Res on the proliferation of the cells were tested by CCK-8assay. The ARPE-19 cells were treated with Res at concentrations of 0,100,150 and 200 μmol /L for 48 h. The effects of Res on the cell cycle and apoptosis were detected by flow cytometry with Annexin V-FITC /PI staining. The protein expression of proliferating cell nuclear antigen（ PCNA） was detected by immunofluorescent assay. The mRNA expression of PCNA,P21 and P27 was determined by real-time PCR. RESULTS： The results of CCK-8 assay showed that Res inhibited the proliferation of ARPE-19 cells in a time- and dose-dependent manner. The treatment with Res for 48 h resulted in an arrest of cell cycle at S phase without increasing cell apoptosis. Res inhibited the protein expression of PCNA in ARPE-19 cells. The results of real-time PCR showed that Res increased the mRNA expression of P21 and P27,and decreased the mRNA expression of PCNA. CONCLUSION： Res inhibits the proliferation of ARPE-19 cells and induces the cell cycle arrest at S phase. The mechanism may be related to up-regulation of P21 and P27,and down-regulation of PCNA.