水稻(Oryza sativa L.)Waxy基因第一内含子+1位G→T突变导致直链淀粉含量降低,针对该位点开发了鉴别两种不同Waxy基因型的标记,通过对143份水稻微核心种质材料进行检测,结果表明,该基因同时影响水稻直链淀粉舍量和胶稠度。其中G型等位基因直链淀粉含量平均值为22.53%,极显著高于T型的8.89%(P〈0.01),而G型材料胶稠度为52.11mm,极显著低于T型(88.33mm)(P〈0.01),并且直链淀粉含量和胶稠度在所有供试材料中表现出极显著负相关(相关系数-0.816,P〈0.01),因此,Waxy基因同时影响水稻直链淀粉含量与胶稠度两个品质相关性状.在改良稻米品质的分子育种中将具有重要的应用价值.
The base substitution (G→T) at +1 loci of the first intron of Waxy gene resulted in the decrease of AC. Identification marker targeted at this SNP loci site of these two different Waxy allele were developed . Results of 143 rice mini-core collections showed that Waxy gene affected amylose content (AC) and gel consistency (GC) simultaneously. The average AC was 22.53% for G-type allele which was significantly higher than that of T-type allele (8.89%). Its GC was significantly lower than that of T-type allele with (52.11 mm and 88.33 mm, respectively). AC was significantly negatively correlated with GC (correlation=-0.816, P〈0.01). Waxy gene affected grain quality traits AC and GC, having significant application in rice molecular breeding.