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中文摘要：
从病区健康花生植株茎秆内分离到1株对花生青枯病菌Ralstonia solanacearum有强拮抗作用的内生细菌,命名为BZ6-1菌株。拮抗试验表明,该菌具有较广抗菌谱,对多种植物病原真菌有较强的抑制作用。形态观察和16SrDNA同源性分析初步鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens。该菌的最佳培养基组成为：糖蜜10g.L-1,蛋白胨0.5g.L-1,酵母膏0.5g.L-1。最适发酵条件为pH8.5、装液量50mL/250mL、温度25℃、转速200r.min-1、培养时间21h,在此条件下拮抗圈直径达34.0mm。田间试验结果表明BZ6-1菌株对花生青枯病防效达62.3%。
英文摘要：
An endophytic bacteria strain BZ6-1,isolated from the stem of healthy peanut plants in Ralstonia solanacearum-infested fields,suppressed R.solanacearum greatly and was of broad spectrum against plant pathogenic fungi.According to its morphology and 16S rDNA sequence,the strain BZ6-1 was preliminarily identified as Bacillus amyloliquefaciens.Diameter of inhibition zone reached 34.0 mm under the optimal fermentation conditions for antibiotic production,which was molasses 10 g,peptone 0.5 g and yeast extract 0.5 g in l L medium at pH 8.5,temperature 25 ℃,rotation rate 200 r·min-1,medium amount 50 mL in 250 mL flask and incubation period 21 h.Field trials demonstrated that the control efficiency of strain BZ6-1 against peanut bacterial wilt disease was 62.3%.
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