中文摘要：

肝糖，葡萄糖的聚合物，是存储精力的一个重要工具。它被肝糖降级 phosphorylase ( GPH )和 hexokinase ( HK )，肝糖 phosphorylase ，和 hexokinase cDNAs ( Ca-GPH 和 Ca-HK ，分别地)，它编码涉及肝糖使用的主要的酶，克隆并且描绘并且过去常在 Crassostrea angulata 在 mRNA 水平调查肝糖新陈代谢的规定。他们的表示侧面在不同纸巾并且在不同繁殖阶段期间被检验。GPH 的全身的 cDNA 是在有开的读框架(ORF ) 预言了编码 868 氨基酸(aa ) 的蛋白质的 2 607 bp 的长度的 3 078 bp。全身的 HK cDNA 是 3 与 1 433 bp 的 ORF， 088 bp 长预言了编码 505 aa 的蛋白质。两基因的表示层次被发现比在披风，鳃，和内脏的质量在性腺和内转肌肌肉显著地更高。他们在内转肌肌肉是特别高级的，它建议这些牡蛎能使用肝糖生产葡萄糖的容易地可得到的供应支持内转肌肌肉活动。两基因的规定也被发现经由 qRT-PCR 并且在 situ 杂交与肝糖内容被相关并且依赖于繁殖周期的阶段(开始，成熟，成熟) 。这样，看来， Ca-GPH 和 Ca-HK 的表达式被牡蛎的繁殖周期驾驶，反映在精力使用的肝糖和在 C 的 gametogenic 开发起的中央作用。angulata。Ca-GPH 和 Ca-HK 能作为有用分子的标记被使用在 C 识别肝糖新陈代谢和复制的阶段，这这里被建议。angulata。

英文摘要：

Glycogen, a polymer of glucose, is an important means of storing energy. It is degraded by glycogen phosphorylase （GPH） and hexokinase （HK）, glycogen phosphorylase, and hexokinase cDNAs （Ca-GPH and Ca- HK, respectively）, which encode the primary enzymes involved in glycogen use, cloned and characterized and used to investigate the regulation of glycogen metabolism at the mRNA level in Crassostrea angulata. Their expression profiles were examined in different tissues and during different reproductive stages. Full-length cDNA of GPHwas 3 078 bp in length with a 2 607 bp open reading frame （ORF） predicted to encode a protein of 868 amino acids （aa）. The full-length HK cDNA was 3 088 bp long, with an ORF of 1 433 bp, predicted to encode a protein of 505 aa. Expression levels of both genes were found to be significantly higher in the gonads and adductor muscle than in the mantle, gill, and visceral mass. They were especially high in the adductor muscle, which suggested that these oysters can use glycogen to produce a readily available supply of glucose to support adductor muscle activity. The regulation of both genes was also found to be correlated with glycogen content via qRT-PCR and in situ hybridization and was dependent upon the stage of the reproductive cycle （initiation, maturation, ripeness）. In this way, it appears that the expression of Ca-GPH and Ca-HK is driven by the reproductive cycle of the oyster, reflecting the central role played by glycogen in energy use and gametogenic development in C. angulata. It is here suggested that Ca- GPH and Ca-HK can be used as useful molecular markers for identifying the stages of glycogen metabolism and reproduction in C. angulata.