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香港巨牡蛎与近江牡蛎杂交及回交子代早期生长发育比较
  • ISSN号:1000-0615
  • 期刊名称:水产学报
  • 时间:2013.8.21
  • 页码:1155-1161
  • 分类:Q321.2[生物学—遗传学] S968.3[农业科学—水产养殖;农业科学—水产科学]
  • 作者机构:[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003, [2]大连海洋大学水产与生命学院,辽宁省贝类良种繁育工程技术研究中心,辽宁大连116023, [3]中国科学院南海海洋研究所,海洋生物资源可持续利用重点实验室,广东广州510301
  • 相关基金:国家自然科学基金项目(31172403); 国家重点基础研究发展规划项目(2010CB126403)
  • 相关项目:牡蛎远缘杂交的杂种优势及遗传机制研究
中文摘要:

为评估香港巨牡蛎与近江牡蛎杂交及回交育种的可行性,以香港巨牡蛎与近江牡蛎为实验对象,比较了两种牡蛎杂交与回交子代的受精率、孵化率、幼虫及稚贝的生长和存活率。结果表明,香港巨牡蛎卵子可以与近江牡蛎精子受精,受精率为47.33%±2.52%,但近江牡蛎卵子与香港巨牡蛎精子不受精。在回交实验中,香港巨牡蛎(H♀)与近江牡蛎(A♂)的杂交牡蛎(HA)与香港巨牡蛎(HH)或近江牡蛎(AA)都可以正常交配,但各回交组受精率明显低于两牡蛎自交组。幼虫期,HA幼虫存活率明显低于两牡蛎自交组HH和AA;HA幼虫生长快于AA,但慢于HH;A♀×HA♂回交组和HA自繁组的幼虫存活率为0。稚贝期,HA稚贝存活率与HH、AA自交组存活率差异不显著。HA稚贝生长快于HH,但慢于AA。另外,研究发现H♀×HA♂回交组幼虫和稚贝的生长与HA差异不显著,优于其它回交组。

英文摘要:

Hybridization and backcrossing between Crassostrea hongkongensis and Crassostrea ariakensis were conducted in this study, and consequently, the experimental groups HH ( C. hongkongensis), AA ( C. ariakensis) ,HA( C. hongkongensis ♀ × C. ariakensis ♂ ), AH( C. hongkongensis ♂ × C. ariakensis ♀ ), HAHA(HA♀ ×HA ♂ ),HHA(HH ♀ ×HA ♂ ),HAH(HA ♀ ×HH ♂ ),HAA(HA ♀ ×AA ♂ ),AHA ( AH ♀ × AA × ) were produced using the method of diallel cross. The results showed that C. hongkongensis eggs can be fertilized by C. ariakensis sperm (47.33 % ±2.52% ), but not vice versa. The fertilization can occur between HA and HH or AA. The fertilization rates of backcrossing groups were lower than those of inbred groups HH and AA. The hatching rates of the backcrossing between HA and AA were lower than those of the other groups, which were 20.33% ±2.08% (AHA) ,55.67% ±2.52% (HAA) respectively. The larval survival rate of the AHA and HAHA was 0 ;The survival of HA larvae was lower than HH and AA. The hybrid larvae grew faster than C. ariakensis larvae, but grew slower than C. hongkongensis. The survival rates of HA, HH, and AA juveniles were not significantly different(P 〉 0.05 ). The hybrid juveniles grew faster than C. hongkongensis larvae, but grew slower than C. ariakensis. In addition, the larval and juvenile growth of HHA and HA was not significantly different(P 〉 0.05 ). The larval survival rate was not significantly different in HHA and HA. The juvenile survival rate of HHA was lower than HA, but was higher than that of the other backcrossing groups. The results provide a basis for study on the hybridization and backcrossing between C. hongkongensis and C. ariakenis.

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期刊信息
  • 《水产学报》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中国水产学会
  • 主编:黄硕琳
  • 地址:上海市军工路334号上海水产大学
  • 邮编:201306
  • 邮箱:jfc@shou.edu.cn
  • 电话:021-61900228
  • 国际标准刊号:ISSN:1000-0615
  • 国内统一刊号:ISSN:31-1283/S
  • 邮发代号:4-297
  • 获奖情况:
  • 曾先后三次获中国科协专项基金资助,获首届《CAJ-CD规范》执行优秀奖,获水产类期刊一等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:26128