中文摘要：

目的观察实验性大鼠关节软骨损伤时血清中吡啶啉水平变化，探讨吡啶啉作为软骨损伤生物标志的意义。方法将40只Wistar大鼠按体质量分为对照组和T-2毒素组，分别喂成品颗粒饲料和经T-2毒素染毒（剂量为100ng／g）的颗粒饲料。于第3、6个月时，光镜检测透明软骨的组织病理改变情况，并用ELISA夹心法检测大鼠血清吡啶啉。结果T-2毒素引起的大鼠关节软骨病理改变具有时间效应，染毒3个月组大鼠关节软骨表层梭形软骨细胞消失，软骨细胞变形、变性、核固缩、排列紊乱；随时间延长，病变加重，染毒6个月组大鼠关节软骨细胞坏死明显，可见大面积无细胞区，关节表面凹陷，不光滑，有的病例软骨细胞出现反应性增生，基质纤维化。T-2毒素组3个月大鼠血清毗啶啉水平[（2．12±0．40）μg／L]较同期对照组（0μg／L）明显升高，两组比较差异有统计学意义（t=11．78，P〈0．01）；染毒6个月大鼠血清吡啶啉水平[（5．30±2．01）μg/L]较同期对照组[（1．95±0．07）μg／L]和同组3个月时明显升高，两两比较差异有统计学意义（t值分别为4．70、4．34．P均〈0．01）。结论大鼠关节软骨病理改变越严重，血清吡啶啉水平越高，血清吡啶啉水平与关节软骨病理损伤呈明显的对应关系，血清吡啶啉可作为关节软骨损伤的生物标志。

英文摘要：

Objective To investigate the changes of pyridinoline level in the serum of rats with articular cartilage damage induced by T-2 toxin, and to explore its significance as the biomarker of articular cartilage damage. Methods Forty Wistar rats were divided into control group and T-2 toxin group according to their body weight, which were fed on commercial grain fodder and grain fodder contaminated with T-2 toxin （100 ng/g）, respectively. At the end of 3, 6 month, the histopathological changes of hyaline cartilage were detected by optical microscope, and the content of pyridinoline in serum was determined by ELISA kits. Results The pathological changes of rat articular cartilage induced by T-2 toxin were in a time-dependent manner, in 3-month T-2 toxin groups, the oval chondroncytes in surface layer disappeared, and all chondrocytes showed distortion, degeneration, karyopyknosis and disarrangement. With the advancement of the experiment, damages were aggravated, extensive necrosis of chondroncytes in rats treated by T-2 toxin for 6 months was found and many blank areas of cells were present. The articular surface was sunken and unsmooth. In some cases, fibrillation of cartilage and reactive hyperplasia of chondrocytes were seen. The pyridinoline level[ （2.12 ± 0.40）μg/L] of rats treated by T-2 toxin for 3 month increased markedly, compared with the 3-month control group（0μg/L）, the difference being significant（t = 11.78, P 〈 0.01）. The pyridinoline level[ （5.30 ± 2.01 ）μg/L] of rats treated by T-2 toxin for 6 month increased markedly, compared with the 6-month control group[ （1.95 ± 0.07）μg/L] and 3-month T-2 toxin group, the difference being significant（t = 4.70,4.34, all P 〈 0.01 ）. Conclusions The pathological damage of rat articular cartilage is more severe when the level of pyridinoline in serum is higher, which suggests that the level of pyridinoline in serum obviously corresponds with the pathological damage of cartilage. So, pyridinoline in serum could be used as a biomark