中文摘要：

目的：探讨慢病毒转染对鼻咽癌细胞株5-8F增殖、迁移的影响,以验证慢病毒转染是否能有效的应用于鼻咽癌细胞的增值及迁移相关研究。方法：以红色荧光标记的慢病毒为转染载体,选定不同的MOI值转染鼻咽癌5-8F细胞株,扩大培养后筛选纯化,流式细胞仪检测转染效率。以最佳MOI值转染后的5-8F（RFP-5-8F）细胞为实验组,未转染的亲代5-8F为空白对照组,取对数生长期未转染的亲代5-8F和红色荧光标记的慢病毒转染的5-8F（RFP-5-8F）细胞进行MTT、划痕实验,观察细胞镜下形态,了解细胞转染前后生长曲线,细胞迁移能力的变化。结果：流式细胞仪检测5-8F细胞慢病毒转染效率大于95%,转染最佳MOI值为30,镜下荧光强度适中。实验组与对照组比较,转染前后5-8F细胞光镜形态相似,生长曲线一致,差异无统计学意义（P=0.997）,划痕实验显示5-8F与RFP-5-8F细胞迁移能力一致,差异无统计学意义（P〉0.05）。结论：慢病毒转染后鼻咽癌细胞能真实有效的的反应原细胞的增值及迁移能力,可以很好的应用于鼻咽癌增殖及其转移机制的相关研究。

英文摘要：

Objective： To explore the effects of lentivirus transfection on the proliferation and migration of Nasopharyngeal carci- noma line 5-8F cells, and to verify whether lentivirus transfection can be effectively applied to the research for the proliferation and mi- gration of Nasopharyngeal carcinoma cell line 5-8F cells. Methods： Different MOI values were selected to transfect Nasopharyngeal car- cinoma line 5-8F cells with red fluorescence-labeled lentivirus as transfection vector. RFP-5-8F cells were cultured and then were puri- fied after transfection. Transfection efficiency was determined by flow cytometry after transfection. RFP-5-8F cells transfected optimal MOI values was considered as the experimental group, and the untransfected parental 5-8F cells for the control group. MTT and Wound healing assays were used to detect the migration and proliferation of 5-8F and RFP-5-8F in logarithmic phase, and the morphology of these cells were observed under microscope. The cell growth curve and migration ability was analyzed. Results： Transfection efficiency of 5-8F cells were higher than 95 % by flow cytometry, and the best MOI was 30 with moderate endoscopic fluorescence intensity. Com- pared with that in the control group, 5-8F and RFP-5-8F cells in the experimental group were morphologically similar under microscope, and the growth curve of both were consistent, without statistical significance （P=0.997）. Wound healing assays showed a consistent 5-8F and RFP-5-8F cell migration ability, without statistical significance （P〉0.05）. Conclusion： NPC ceils after lentivirus transfection could ef- fectively maintain their ability of proliferation and migration and could be applied to related mechanical research on the proliferation and metastasis of NPC.