中文摘要：

目的 探讨白藜芦醇对动脉粥样硬化兔血清中炎症因子白细胞介素（IL）-1β、IL-6、肿瘤坏死因子-α（TNF-α）,以及核因子-κB（NF-κB）、丝裂原活化蛋白激酶（MAPK）信号通路的影响,明确白藜芦醇抗动脉粥样硬化的机制.方法 将雄性纯种日本大耳白兔以完全随机区组法分为6组,即正常对照组（对照组,n=10）,高脂模型组（高脂组,n=10）,白藜芦醇低、中、高剂量组（n均为10）,白藜芦醇预防组（预防组,n=10）.酶联免疫分析（ELISA）法检测血清炎性指标IL-1 β、IL-6、TNF-α的表达水平,蛋白免疫印迹（Western blot）法检测主动脉组织中NF-κB、p38丝裂原活化蛋白激酶（p38MAPK）、细胞外调解激酶（ERK 1/2）和应激活化蛋白激酶（JNK）的蛋白表达水平.结果 高脂组IL-1β、IL-6、TNF-α浓度均显著高于对照组（P均＜0.05）.白藜芦醇低、中、高剂量组及预防组IL-1β、IL-6、TNF-α浓度则均低于高脂组（P均＜0.01）,且此作用呈剂量依赖性.此外,高脂组主动脉组织中NF-κB、p38MAPK、JNK、ERK1/2蛋白磷酸化水平均高于对照组（P均＜0.05）.白藜芦醇低、中、高剂量组及预防组NF-κB、p38MAPK、JNK蛋白磷酸化水平则均低于高脂组（P均＜0.05）,ERK1/2蛋白磷酸化水平与高脂组比较差异无统计学意义.结论 白藜芦醇可以降低动脉粥样硬化兔血清炎症因子水平,其作用机制可能与抑制NF-κB、MAPK信号通路的激活有关.

英文摘要：

Objective Inflammation serves as the initial pathologic step of cardiovascular diseases including atherosclerosis.Resveratrol possesses many pharmacological properties including antioxidant,cardioprotective and anti-cancer effects.In this study,we investigate the anti-inflammatory effect and mechanisms of resveratrol in an atherosclerotic rabbit model.Methods Rabbit were assigned to six groups （n =10 each）：control,high fat diet group,resveratrol low,medium and high dose groups,resveratrol pretreatment group.The serum tumor necrosis factor-α （TNF-α）,interleukin-6 （IL-6） and intedeukin-1β （IL-1β） were analyzed by Enzyme-linked immuno sorbent assay （ELISA）.Phosphorylation levels of mitogen-activated protein kinases （MAPKs） cascades and NF-κB were determined by Western blot.Results Compared with the control group,the expression of serum inflammatory factors IL-1β,IL-6,TNF-α were increased in high-fat group （all P 〈 O.05）.Compared with high-fat group,the expressions of IL-6,IL-1β,TNF-α were significantly reduced in resveratrol low,medium,high dose groups and resveratrol pretreatment group （all P 〈 0.01）,and this effect is dose-dependent.In addition,the NF-κB,p38MAPK,JNK,ERK1/2 protein phosphorylation in high-fat group were significantly upregulated compared with control group （P 〈0.05）,which （except ERK1/2 phosphorylation level） were significantly downregulated in resveratrol treatment group and resveratrol pretreatment group.Conclusion This study indicates that resveratrol .