中文摘要：

目的观察辛通畅络法复方肾苏Ⅱ对局灶节段性肾小球硬化（FSGS）大鼠肾间质纤维化（RIF）进程及转化生长因子β1（TGF-β1）表达的影响。方法48只健康雄性SD大鼠采用随机数字表法分为对照组12只和造模组36只。造模组大鼠采用阿霉素注射法诱发FSGS型阿霉素肾病模型，再采用随机数字表法均分为模型组、西药组和中药组，西药组0.33mg/100g灌服洛汀新混悬液，中药组3.5g/100g灌服肾苏Ⅱ，模型组及对照组灌服等量生理盐水，共12周。行HE染色、Masson染色观察各组肾小管-间质病理形态改变情况，测定肾小管间质损伤指数和肾小管-间质纤维化指数，并采用免疫组化SP法检测肾小管间质纤维连结蛋白（FN）和TGF-β1相对表达水平。结果中药组FSGS大鼠肾间质纤维化进程延缓，肾小管-间质损伤指数（1.51±0.80）低于模型组（2.18±0.38）和西药组（1.79±0.24），肾小管-间质纤维化指数（2.39±0.13）低于模型组（3.11±0.25）和西药组（2.80±0.41），肾间质FN相对表达量（14.19±3.06）低于模型组（21.25±3.31）和西药组（18.51±2.29），TGF-β1相对表达量（2.64±0.21）低于模型组（6.02±0.12）和西药组（3.79±0.46），差异均有统计学意义（均P＜0.05）。结论辛通畅络法复方肾苏Ⅱ可延缓FSGS大鼠肾间质纤维化进程，作用机制可能与抑制TGF-β1表达有关。

英文摘要：

Objective To observe and discuss the effect of the traditional Chinese drug complex prescription ShensuⅡfrom Xintong Changluo therapeutic principle on renal interstitial fibrosis (RIF) and transforming growth factor-β1 (TGF-β1) expresssion in focal segmental glomerulosclerosis (FSGS) model rats. Methods Forty-eight healthy male SD rats were randomly divided into control group (n=12) and modeling group (n=36). Rats of modeling group were injected by doxorubicin hydrochoride for FSGS model. Rats of modeling group were sub-divided into model group, benazepril group and TCM group randomly. In 12 weeks, TCM group was given by intragastric administration of ShensuⅡ (3.5 g/100 g), benazepril group was given by intragastric administration of benazepril suspension (0.33 mg/100 g), control group and model group were given by intragastric administration of same volume of saline. HE /Masson staining was used to observe changes of tubulointerstitial pathomorphology. The degree of injury and fibrosis was measured. The expressions of fibronectin (FN) and TGF- β1 were detected by immunohistochemical SP method. Results The process of renal interstitial fibrosis was slower in FSGS rats of TCM group. Renal interstitial pathological index was 1.51±0.80 in TCM group, which was lower than that of model group(2.18±0.38) and benazepril group (1.79±0.24). The index of renal interstitial fibrosis was 2.39±0.13 in TCM group, which was lower than that of model group (3.11±0.25) and benazepril group (2.80±0.41). The relative expression of FN in renal interstitial was 14.19±3.06 in TCM group, which was lower than that of model group (21.25±3.31) and benazepril group (18.51±2.29). The relative expression of TGF-β1 in renal interstitial was 2.64±0.21 in TCM group, which was lower than that of model group (6.02±0.12) and benazepril group (3.79±0.46). All the differences were statistically significant (P<0.05).Conclusion Xintong Changluo complex prescription ShensuⅡcan reduce the process of renal interstitial fibrosis in FSG