为了解光质及内源激素对黄瓜幼苗形态建成的影响,并向设施栽培育苗过程中光源的合理配置提供理论依据,采用发光二极管(Light—emittingdiode,LED)精确调制光谱能量分布,以白光为对照(CK),研究红、蓝、黄、绿4种单色光质下黄瓜幼苗叶片和下胚轴中赤霉素(GA、)、生长素(1AA)和脱落酸(ABA)含量的变化.结果表明:不同光质可能通过调节内源激素的分布来影响黄瓜幼苗的光形态建成.红光和蓝光通过影响IAA的极性运输,影响真叶的生长进程,其中红光显著促进了IAA的极性运输,叶片中IAA含量仅220μgg-1,而下胚轴中IAA含量却高达120μgg-1;黄光处理下叶片中GA,含量高(约为对照的2倍),抑制叶片脱黄化,并引起叶片卷曲.各单色光质下黄瓜幼苗下胚轴与对照相比均有不同程度伸长,说明光对幼苗下胚轴的抑制作用可能是各种光质共同作用的结果.黄、绿光处理下胚轴中GA,含量显著升高,ABA含量显著降低,下胚轴冠著伸长;红光引起下胚轴中GA,和ABA含量显著升高,但下胚轴长度增加不明显,黄瓜幼苗下胚轴的伸长可能与光质调节GA;和ABA的相对水平密切相关.综上所述,不同光质对黄瓜幼茁中内源激素含量的变化影响显著,从而引起了幼苗形态特征的显著变化,图5参24
To investigate the effect of monochromatic LED light quality on endogenous hormone content in leaves and hypocotyl of cucumber seedlings, the endogenous GA3, 1AA and ABA in seedling leaves and hypocotyl under red, blue, yellow and green lights were determined by High Performance Liquid Chromatograph (HPLC). The light-emitting diodes (LEDs) were used as optical source and cucumber seedlings as experimental material. The experimental results showed that light qualities influenced the photomorphogenesis of cucumber seedlings by regulating the distribution of endogenous hormone. Red light increased polar 1AA transport to hypocotyl with the content of 1AA in leaves being 20 pg g~ but 120 pg g-~ in hypocotyls. Contrarily, blue light promoted the accumulation of massive IAA in leaves to inhibit the euphylla growth. Yellow light curled the leaves, and at the same time, failed in de-etiolating and increasing the GA3 concentration in leaves ( about twice as much as control). The hypocolyls under all monochromatic lights were longer than that of the control treatment, suggesting that every monochromatic light had its function on repressing hypocotyl extension. Yellow light and green light enlongated the hypocotyl dramaticlly due to high GA3 concentration and low ABA concentration in hypocotyl. Red light did not significantly elongate the hypocotyl due to high GA3 and ABA concentrations. In conclusion, light quality significantly affected the content of endogenous hormone of cucumber seedlings, and then induced changes of morphological characteristics of seedlings. These results may lay a theoretical foundation for setting reasonable light conditions for cultivated seedlings.