中文摘要：

目的：探讨雌激素受体α与β亚型在不同年龄段小鼠胸腺内的表达。方法：免疫组化分析雌激素受体α与β亚型在3、8及15月龄段小鼠胸腺内的表达水平;双色免疫荧光分析雌激素受体α与Treg细胞标志Foxp3表达的关系。结果：3月龄小鼠的胸腺皮质区及髓质区内有大量的雌激素受体β及α亚型的阳性细胞。然而,8月龄及15月龄的小鼠胸腺则缺乏雌激素受体β亚型表达而雌激素受体α亚型阳性细胞仍然可发现于这些胸腺的皮质区内。使用雌激素受体α/β及Foxp3（调节性T细胞的标志）抗体对标本进行双色标记结果证实雌激素受体α/β阳性的细胞为Foxp3阴性。结论：成年小鼠胸腺缺乏雌激素受体β,但雌激素受体α为阳性,提示雌激素受体α是介导雌激素引发的胸腺萎缩的关键激素受体。同时也证实雌激素受体α/β不参与Foxp3调节性T细胞的发育。

英文摘要：

Objective：To detect estrogen receptor α and β（ER-α,ER-β） protein expression in different age of mouse thymus.Methods：Protein expression of ER-α and ER-β in thymus was analyzed via immunohistochemistry.Moreover,the relationship between ER-α and cytokeratin 18（epithelial cell marker） was further tested through fluorescence double-staining.Results：Immunohistochemical analysis revealed that both ER-α and β protein was found in nuclei of some thymocytes of 3 month mice.However,expression of ER-β was absence while ER-α was still positive in aged mice,such as 12 months and 16 months old.Double staining further confirmed that lots of ER-α/β positive cells were Foxp3 negative cells.Conclusion：Expression of ER-β is absent while ER-α is still positive in thymus of aged mice,which indicates ER-α is the critical estrogen receptor that involves in thymic involution.Moreover,ER-α/β do not participate in Treg development within thymus.