中文摘要：

通过RNA亲和层析（RNA affinity chromatograph），4种可能与人PSF（humanpolypyrimidine tract—bindingprotein—associateds plicing factor，hPSF）蛋白结合的长非编码RNA（long non—coding RNA，lncRNA）片段在人黑色素瘤细胞yusac细胞核RNA文库中被筛选得到．它们分别定位于人内源性逆转座子L1PAl6、MERllC、非编码基因MALAT-1以及一个未知基因（unknown gene）．紫外交联分析（UV crossqinking assay）证明在体外条件下这4种lncRNA片段均能与PSF蛋白结合；RNA免疫沉淀（RNA immunoprecipitation，RNA—IP）进一步证实在体内条件下这4种1ncRNA均能与hPSF发生相互作用．最后，半定量R’F—PCR表明，未知lncRNA特异高表达于人黑色素瘤细胞，其余3种lncRNA在多种人类肿瘤细胞中的丰度均较正常细胞明显升高．

英文摘要：

Four hPSF （human polypyrimidine tract-binding protein-associated splicing factor）-binding lncRNA （long non-coding RNA） fragments were isolated by RNA affinity chromatograph from a nuclear RNA library of human yusac melanoma cell line. They map to retrotransposon L1PA16 and MERllC, non-coding gene MALAT-1 and an unknown gene, respectively. UV cross-linking assay confirmed that the four lncRNAs bind to hPSF in vitro. RNA-immunoprecipitation （RNA-IP） demonstrated that they also form lncRNA/hPSF protein complexes in vivo. A screen of 9 human tumor cell lines by semi-quan titative RT-PCR shown that the lines. The other three lncRNAs cells. unknown lncRNA was exclusively expressed in human melanoma cell were also overexpressed in human tumor cells in contrast to normal