中文摘要：

为了安全便捷地获取严重急性呼吸系统综合征冠状病毒（Severe Acute Respiratory Syndrome coronavirus,SARS coronavirus）的RdRp基因,作者合成了26条寡聚核苷酸片段,利用组装PCR（assembly PCR）在体外构建了SARS病毒的RdRp基因片段,并建立了基于Vero E6细胞的SARS RdRp基因的稳定表达株.之后设计了4对针对SARS RdRp基因的siRNA,基因干扰表明这4对siRNA均能高效干扰SARS RdRp基因在Vero E6细胞中的表达.至此,本文提供了一种安全便捷地获取病毒基因的方法.

英文摘要：

To get SARS coronavirus from DNA shuffling was established sembled from 26 o stable expression ce NAs against 645bp- showed that all the rent research therefore RdRp gene conveniently, and a 645bp fragment of Rd an assembly PCR proceduredeveloped Rp gene of SARS coronavirus was as- gosin vitro. Thereafter,the 645bp-RdRp was transfected into Vero E6 lls, Vero-RdRp, were selected out for RNA interference test. Four syn RdRp were used to interfere RdRp RNA transcribed from Vero-RdRp. four siRNAs decreased the expression of the assembled RdRp efficient ore provided an efficient strategy to generate the access-limited virus ge cells and the thesized siR- The results ly. The cur- nes in vitro.