中文摘要：

目的：探讨雷公藤红素对破骨细胞分化过程中趋化因子CCl4表达的影响。方法：采用100 ng/m L M-CSF,50 ng/m L RANKL诱导大鼠骨髓源性破骨细胞,72 h后用PCR的方法检测趋化因子CC家族的表达。用CCK-8的方法检测雷公藤红素对骨髓细胞增殖的影响。以不同浓度的雷公藤红素（0.1、0.25、0.5μmol/L）干预大鼠骨髓源性破骨细胞,用PCR的方法检测CCl4的表达,TRAP染色鉴定破骨细胞。结果：在大鼠骨髓源性破骨细胞形成过程中,早期（第3天）趋化因子CCl2、CCl4、CCl7、CCl8均明显上调,其中CCl4表达增多的最为明显。雷公藤红素在0.1～0.5μmol/L范围内,浓度依懒性地抑制大鼠骨髓破骨细胞的分化,在0.1～0.5μmol/L时对破骨细胞的抑制作用最强,同时下调趋化因子CCl4表达。结论：雷公藤红素抑制趋化因子CCl4的表达,减少破骨细胞分化。

英文摘要：

Objective：To observe the effect of Celastrol on the expressions of CCl4 in osteoclast differentiation.Methods：Rat bone marrow was stimulated with 100 ng / m L M-CSF and 50 ng / m LRANKL.After 72 h,the expressions of factor CC chemokine family were assayed by real-time PCR and immunofluorescence method.Celastrol on the proliferation of bone marrow cells was detected by the method of CCK-8.Different concentrations of Celastrol（0.1 μmol / L,0.25 μmol / L,0.5 μmol / L） intervened the rat bone marrow-derived osteoclasts.The expressions of CCl4 were detected by PCR and osteoclasts identified by TRAP.Results：The expressions of CCl2,CCl4,CCl7 and CCl8in bone marrow-derived osteoclasts were significantly increased and the expression of CCl4 most obviously increased.Celastrol（0.1-0.5 μmol / L） could inhibit the differentiation of rat bone marrow cells in a dose-dependent manner and reduce expression of CCl4.Conclusion：Celastrol could inhibit the expression of CCl4 and reduce osteoclast differentiation.