中文摘要：

目的：观察雷公藤红素（Celastrol,Cel）对类风湿关节炎滑膜成纤维细胞系MH7A细胞中核因子κB受体活化因子配体（RANKL）、骨保护素（OPG）、白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）及白细胞介素-8（IL-8）表达的影响。方法：以白细胞介素-1β（IL-1β）刺激MH7A,不同浓度的Cel（0.1、0.5、1.0、2.0μmol/L）干预细胞24 h后,采用real-time PCR和免疫荧光的方法检测RANKL、OPG、IL-6、TNF-α及IL-8的表达。结果：IL-1β刺激MH7A细胞系24 h后,RANKL、OPG、IL-6、TNF-α及IL-8mRNA及RANKL免疫荧光明显增强,Cel呈剂量依赖性抑制MH7A中IL-1β诱导的RANKL、OPG、IL-6、TNF-α及IL-8 mRNA水平（P〈0.05）和MH7A中RANKL免疫荧光表达,显著上调OPG/RANKL轴比值。结论：Cel能调节滑膜成纤维细胞中OPG/RANKL轴和抑制促炎因子、趋化因子的表达,可能在阻止类风湿关节炎＂炎症＂和＂骨侵蚀＂中发挥重要作用。

英文摘要：

Objective：To observe the effect of Celastrol on the expressions of RANKL,OPG,IL-6,TNF-α and IL-8 in human rheumatoid synoviocyte MH7A.Methods：MH7A was stimulated with IL-1β and added in different concentration of Celastrol（0.1,0.5,1,2 μmol/L）.The expressions of RANKL,OPG,IL-6,TNF-α and IL-8 in MH7A were assayed by real-time PCR and immunofluorescence.Results：The expressions of RANKL,OPG,IL-6,TNF-α and IL-8 in MH7A were significantly increased in MH7A upon stimulation with IL-1β（P〈0.05）.Celastrol could inhibit the expressions of RANKL,OPG,IL-6,TNF-α and IL-8 in a dose-dependent manner and notably elevated the ratio of OPG/RANK axis.Conclusion：Celastrol could regulate the ratio of OPG/RANKL axis and suppress the inflammatory cytokines and chemokine expression in rheumatoid arthritis synovioblast,suggesting a potent role in preventing the inflammation and bone erosion in rheumatoid arthritis.