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氨基甲酸酯类农药适体筛选技术的建立
  • 时间:0
  • 分类:TS207.5[轻工技术与工程—食品科学;轻工技术与工程—食品科学与工程]
  • 作者机构:[1]Key Laboratoy. of Food Quality and Safety of Jiangsu Province, Nanjing 210014, China, [2]Michigan State Universities, East Lansing 48824-1212, USA
  • 相关基金:Project supported by the National Natural Science Foundation of China (No. 30871658) and the "948" Project of the Ministry of Agri- culture of China (Nos. 2011-Z46 and 2011-G5-7)
  • 相关项目:抗体库筛选制备农药通用结构抗独特型抗体研究
中文摘要:

在最近的年里,有病与三聚氰胺联系了的忍受的食物的二报导爆发。在牛奶的三聚氰胺和它的相关混合物的存在,喂,并且另外的食物导致了对察觉和沾染物的这个班的精确 quantification 的对可靠方法的需要。为在一个复杂矩阵的三聚氰胺的样品预告的处理通常由极的溶剂包含液体抽取,与稳固的阶段抽取由进一步的大扫除列在后面。三聚氰胺和相关混合物的分析被液体或与集体 spectrometry 结合的煤气的色析法的方法通常执行。另外的创新屏蔽方法,使用抗体,分子地印了聚合物,毛状的电气泳动,和金 nanoparticles,也被用来开发试金和生物传感器到三聚氰胺。然而,许多被矩阵效果,三聚氰胺氰尿酸酸建筑群的溶解度,和背景污染这些方法妨碍了。这篇文章为检测三聚氰胺考察最近的开发并且讨论未来方向。

英文摘要:

In recent years, there were two reported outbreaks of food borne illness associated with melamine. The presence of melamine and its related compounds in milk, feed, and other foods has resulted in the need for reliable methods for the detection and accurate quantification of this class of contaminants. The sample pretreatment for melamine in a complex matrix usually involves a liquid extraction by a polar solvent, followed by a further clean-up with solid phase extraction. Analyses of melamine and related compounds are commonly carried out by liquid or gas chromatographic methods conjugated with mass spectrometry. Other innovative screening methods, which use antibodies, molecularly imprinted polymers, capillary electrophoresis, and gold nanoparticles, are also used to develop assays and biosensors to melamine. However, many of these methods have been hindered by matrix effects, the solubility of melamine-cyanuric acid complex, and background contamination. This article reviews recent develop- ments for detecting melamine and discusses future directions.

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