中文摘要：

为制备微囊藻毒素（MCYST-LR）多克隆抗体F（ab′）2片段的方法。采用Protein G亲和柱纯化微囊藻毒素多克隆抗体,经木瓜蛋白酶在温和条件下酶切抗体,利用DEAE-52离子交换树脂柱分离抗体酶切片段,获得F（ab′）2片段。SDS-PAGE分析表明,MCYST-LR多克隆抗体得到有效纯化,木瓜蛋白酶酶切后出现明显F（ab′）2片段的对应谱带;利用紫外分光光度计法测得F（ab′）2片段的产率为30.6%。采用木瓜蛋白酶法成功制备了MCYST-LR多克隆抗体的F（ab′）2片段。

英文摘要：

The polyantibodies against microcystin-LR were purified using affinity chromatography with Protein G.The purified polyclonal antibodies were digested by papain under mild conditions.The antibody fragments were separated by DEAE-52 ion-exchange chromatography to retrieve the F（ab′）2 fragments.The SDS-PAGE results showed that the microcystin-LR polyclonal antibodies effectively purified by using Protein G affinity chromatography released the F（ab′）2 fragments from papain-digested microcystin-LR polyclonal antibodies.The yield rate of F（ab′）2 fragments was 30.6% by UV-spectrophotometer.F（ab′）2 fragments of polyclonal antibodies against MCYST-LR were produced successfully by papain enzymolysis method.