中文摘要：

对野桑蚕CYP30581V1基因进行克隆和序列分析的结果显示，野桑蚕CYP30581V1基因的ORF为1464nt，编码487aa；同源性分析表明，在DNA水平上野桑蚕CYP30581V1基因与家蚕CYP30581基因的同源性达99％，与推导的氨基酸序列完全一致。通过和NCBI中家蚕基因组数据比对和拼接，预测野桑蚕CYP30581V1基因结构中至少含有6个内含子，且内含子与外显子之间的连接符合GT-AT法则。

英文摘要：

The CYP305B1V1 gene from Bombyx mandarina M. was cloned and sequenced. The results showed that the length of ORF of CYP305B1V1 is 1 464 nt, which encodes 487 aa, Homologous analysis showed that the DNA homology of CYP305B1V1 of silkworm and wild silkworm reaches 99%, which is consistent with deduced amino acid sequences. We presume that CYP305B1V1 of wild silkworm contains at least 6 introns and they all obey the GT-AG rule by searching and aligning the silkworm genome database on NCBI.