对老挝家蚕品种L11的线粒体DNA(mitochondrial DNA,mtDNA)cox3基因(cox3)进行PCR扩增、克隆和序列分析。结果表明,在测定的909bp的片段中,含有ATPase6基因及tRNA-Gly基因的部分序列和cox3全序列。其中,cox3读码框包括789个核苷酸,编码262个氨基酸。L11 cox3和已经公布的家蚕(中国家蚕品种夏芳、日本家蚕Aojuku、韩国家蚕Backojam)mtDNAcox3基因完全一致,但与日本野桑蚕、中国野桑蚕的氨基酸同源性分别为99%和98%。
The mtDNA cox3 gene of L11 was amplified , cloned sequenced and analysed. The result showed that partial ATPase 6 tRNA-Gly and complete cox3 gene were contained in 909 bp segment. The open reading frame of cox3 gene contains 789 nucleotides encoding a putative protein of 262 amino acids. The cox3 gene of mtDNA in L11 was completely coincident with that of published Bombyx mori (Chinese Bombyx mori XiaFang, Japanese Bombyx mori Aojuku, Korean Bombyx mori Backojam), but Japanese Bombyx mandarina, Chinese Bombyx mandarina were respectively 99% and 98%.