中文摘要：

目的：观察夏枯草总三萜（TTP）对血小板衍生因子（PDGF-BB）干预的肝星状细胞（HSCT6）中TGF-β1/Smad通路调控以及其抑制HSC增殖的作用。方法：用不同浓度的TTP（10、30、100、300、1 000μg/mL）及PI3K抑制剂（LY294002,10μmol/L）对PDGF-BB刺激的HSC-T6进行处理,四甲基偶氮唑蓝（MTT）法检测细胞增殖;RT-PCR和Western blot法检测HSC-T6中TGF-β1信号转导通路下游中介分子smad2、smad3、smad7表达。结果：TTP给药组可抑制PDGF诱导的HSC-T6增殖,下调smad2、smad3表达,升高smad7表达。结论：TTP对PDGF-BB刺激的HSC-T6增殖具有较好的抑制作用,其机制可能与阻断TGF-β1/Smad信号通路传导有关。

英文摘要：

AIM： To investigate the inhibitory role of total triterpenoids of prunella vulgaris L.（ TTP） in the proliferation,and regulatory effects on the TGF-β1/ Smad pathway in hepatic stellate cellT6（ HSC-T6） stimulated by PDGF. METHODS：HSC-T6 cells were stimulated by PDGF-BB and treated with different does of TTP（ 10,30,100,300, 1000 μg / m L） and PI3 K inhibitor（ LY294002,10 μmol/L）,the HSC-T6 proliferation was detected by MTT,the expression of mRNA and protein levels of smad2,smad3,smad7 were detected by RT-PCR and Western blot. RESULTS：As compared with the model group,TTP could inhibit HSC-T6 stimulated by PDGF-BB. Furthermore,TTP reduced the mRNA and protein expression of smad2,smad3 and increased smad7. CONCLUSION： TTP can inhibit the proliferation of HSC stimulated by PDGF-BB, the mechanism of this process may be related to the interference with TGF-β1/ Smad signal transduction pathway.