中文摘要：

目的 通过检测在大鼠肝纤维化模型及转化生长因子-β1（TGF-β1）刺激的大鼠肝星形细胞（HSC）T6中结节性硬化症（TSC）相关蛋白错构素（hamartin）的表达,并检测HSCT6细胞TSC1基因启动子甲基化状态,探讨肝纤维化疾病治疗的新靶点。方法 构建大鼠肝纤维化模型,培养HSC T6细胞。利用Western blot法检测hamartin蛋白表达,利用焦磷酸测序检测TSC1启动子甲基化。结果 与正常对照组比较,肝纤维化模型组TSC1蛋白表达量较小（P〈0.01）,且TSC1基因启动子甲基化程度较高。结论 TSC1可能与肝纤维化形成有一定关联。

英文摘要：

Objective To explore the expression and the promoter methylation status of the TSC1 in HSC T6 differentiation induced by transforming growth factor β1 （ TGF- β1 ） in vitro, and the expression of the TSC1 in CC14-induced liver fibrosis in rat. Methods Western blot was used to detect the expression of the TSCI. Pyrosequencing was used to test the methylation status of the TSC1 promoter. Results The expression of TSClwas significantly reduced in response to TGF-β1 and CC14（P 〈0. 01 ）, and the TGF- β1 treated group TSC1 promoter was found to be higher methylated than the control group. Conclusion A novel evidence has been provided that TSC1, which may be affected by DNA methylation in TGF- β1 treated HSC T6, may play a vital role in liver fibrosis.