中文摘要：

目的 ：观察过氧化物还原酶4（peroxiredoxin 4,Prdx4）蛋白表达水平的改变对人宫颈癌He La细胞增殖和凋亡的影响。方法：采用脂质体瞬时转染法将表达质粒pc DNA3.0-HA-Prdx4（以pc DNA3.0-HA空载体为阴性对照）和针对人Prdx 4基因的3种小干扰RNA（small interference RNA,si RNA）（以阴性对照si RNA为阴性对照）分别转染宫颈癌He La细胞;用蛋白质印迹法证实Prdx4蛋白表达水平被改变后,采用MTT法和FCM法分别检测He La细胞的增殖活性和凋亡情况。结果 ：pc DNA3.0-HA-Prdx4质粒转染组He La细胞的Prdx4蛋白表达水平明显上调（P〈0.05）,而3个Prdx4 si RNA干扰组的Prdx4表达水平均明显下调（P值均〈0.05）。Prdx4过表达组He La细胞的增殖能力与空白对照组及空载体转染组比较,未发生明显改变（P值均〉0.05）,且凋亡细胞百分率差异也无统计学意义（P值均〉0.05）;而3个Prdx4表达干扰组He La细胞的增殖能力较阴性对照组均明显降低（P值均〈0.05）,凋亡细胞百分率均明显提高（P值均〈0.05）。结论 ：Prdx4蛋白表达水平上调对He La细胞的增殖和凋亡没有明显影响,但其下调会抑制He La细胞增殖并促进细胞凋亡。推测Prdx4有可能成为临床治疗宫颈癌的一个潜在靶点。

英文摘要：

Objective：To investigate the effects of change in expression level of peroxiredoxin 4（Prdx4） protein on proliferation and apoptosis of cervical cancer HeLa cells.Methods：The combination plasmid pcDNA3.0-HA-Prdx4（the empty vector pcDNA3.0-HA was used as the negative control） and three small interference RNAs（siRNAs） against human Prdx4 gene（the negative control siRNA was used as the negative control） were transfected into HeLa cells,respectively.The change in the expression of Prdx4 protein was validated by Western blotting,then MTT assay and flow cytometry（FCM） were performed to detect the proliferation activity and apoptosis of HeLa cells,respectively.Results：The expression level of Prdx4 protein was up-regulated in HeLa cells after transfection with pcDNA3.0-HA-Prdx4 plasmid（P 0.05）,whereas it was down-regulated in HeLa cells after transfection with three Prdx4 siRNAs（P 0.05）.The proliferation activity and apoptotic rate of HeLa cells with Prdx4 protein overexpression were not significantly changed as compared with those of the blank control（without transfection） and the negative control（transfected with pcDNA3.0-HA） groups（all P 0.05）.But three siRNAs-mediated interference of Prdx4 protein expression significantly decreased the proliferation activity and increased the apoptotic rate of HeLa cells as compared with the negative control group（transfected with the negative control siRNA）（all P 0.05）.Conclusion：Although the up-regulation of Prdx4 expression has no effect on the cell proliferation and apoptosis of HeLa cells,the down-regulation of Prdx4 expression can inhibit the cell proliferation and promote the apoptosis.These findings indicate that Prdx4 gene may become a candidate molecular target for cervical cancer therapy.