中文摘要：

目的：探讨上调或沉默过氧化物还原酶4（peroxiredoxin 4,Prdx4）蛋白表达对人肺腺癌A549细胞增殖、凋亡和迁移的影响。方法：利用脂质体瞬时转染法将重组质粒pcDNA3.0-HA-Prdx4（以空载体pcDNA3.0-HA为对照组）和特异性针对人Prdx4基因的siRNA（Prdx4-siRNA）（以阴性对照-siRNA为对照组）分别转染A549细胞;采用实时荧光定量PCR和蛋白质印迹法分别检测Prdx4 mRNA和蛋白的表达水平;CCK-8法和免疫荧光技术分别检测A549细胞的增殖和凋亡情况;细胞划痕实验和Transwell小室法检测A549细胞迁移能力的改变。结果：转入重组质粒pcDNA3.0-HA-Prdx4的A549细胞中Prdx4 mRNA和蛋白的表达水平明显上调（P值均〈0.05）,转入Prdx4-siRNA的A549细胞中Prdx4 mRNA和蛋白的表达水平明显下调（P值均〈0.05）。CCK-8检测结果显示,Prdx4过表达的A549细胞在24、48和72 h时的D450 nm值均明显高于对照组（P值均〈0.05）,并且96 h时的D450 nm值（2.241±0.068和1.596±0.103）差异更加明显（P〈0.01）;沉默Prdx4表达后A549细胞在48、72和96 h时的D450 nm值明显低于对照组（P值均〈0.01）。免疫荧光结果显示,Prdx4过表达的A549细胞在顺铂刺激24和48 h后凋亡细胞数与对照组相比明显减少,差异均具有统计学意义（P〈0.05和P〈0.01）;沉默Prdx4表达的A549细胞在顺铂刺激24和48 h后,凋亡细胞数明显多于对照组（P〈0.05和P〈0.01）。划痕实验结果显示,Prdx4过表达A549细胞的愈合率明显高于对照组（P〈0.01）;沉默Prdx4表达的A549细胞的愈合率明显低于对照组（P〈0.01）。Transwell迁移实验结果显示,Prdx4过表达的A549细胞穿过小室膜的细胞数明显多于对照组（P〈0.01）;沉默Prdx4表达的A549细胞穿过小室膜的细胞数明显少于对照组（P〈0.01）。结论：Prdx4可促进A549细胞的增殖和迁移并抑制其凋亡,其有可能成为临床治疗肺腺癌的一个潜在靶点。

英文摘要：

Objective： To investigate the effects of up-regulating or silencing peroxiredoxin 4 （Prdx4） protein expression on the proliferation, apoptosis and migration of human lung adenocarcinoma A549 cellsMethods： The recombinant plasmid pcDNA3.0-HA-Prdx4 and specific siRNA targeting human Prdx4 gene （Prdx4-siRNA） were transfected into A549 cells by liposome, respectively; while the empty vector pcDNA3.0-HA and the negative control-siRNA were used as the controls. The expression levels of Prdx4 mRNA and protein were detected by real-time fluorescent quantitative-PCR and Western blotting, respectively. The proliferation and apoptosis of A549 cells were detected by CCK-8 assay and immunofluorescence assay, respectively. The migration ability of A549 cells was detected by wound-healing test and Transwell chamber assay.Results： The expression levels of Prdx4 mRNA and protein were up-regulated in A549 cells transfected with the recombinant plasmid pcDNA3.0-HA-Prdx4 （both P 〈 0.05）, while they were down-regulated in A549 cells transfected with Prdx4-siRNA （both P 〈 0.01）. The proliferation ability （D4s0nm） of A549 cells with Prdx4 over-expression was higher than that in the control group at 24, 48 and 72 h （all P 〈 0.05）, and the difference at 96 h was most obvious （P 〈 0.01）. The Das0 nrn values of A549 cells with Prdx4 gene-silencing were decreased （all P 〈 0.01） as compared with the control group at 24, 48, 72 and 96 h. After treatment with cisplatin for 24 and 48 h, the number of apoptotic cells in Prdx4 over-expression group was significantly decreased as compared with the control group （P 〈 0.05 and P 〈 0.01）, while it was significantly increased in Prdx4 gene-silencing group as compared with the control group （P 〈 0.05 and P 〈 0.01）. The wound healing rate in Prdx4 over-expression group was higher than that in the control group （P 〈 0.01）, while it was lower in Prdx4 gene-silencing group than that in the control group （P 〈 0.01）. The number of A5