中文摘要：

目的：构建雄激素受体（AR）功能表位表达载体，为深入研究AR与LRP16体外相互作用机制奠定基础。方法：用真核表达质粒pCMV—AR为模板进行常规PCR扩增，产物纯化后将连入pGEM—T载体，挑取阳性克隆扩增培养后测序并进行酶切鉴定；回收酶切后AR目的片断最终与BamHⅠ／XhoⅠ双酶切的pcDNA3．1（＋）载体相连接。结果：测序结果表明四个AR功能表位表达载体插入片段与GenBank中报道的序列完全一致。结论：在克隆AR基因的基础上，成功构建了一组雄激素受体AR功能表位表达载体，可以用于AR与LRP16在体外转录翻译方面的研究。

英文摘要：

Objective： To construct recombinant expression vectors for function epi-position of androgen receptor so as to provide foundation for further investigation and analysis interaction mechanism in vitro beween LRP16 and AR. Methods： The plasmid pCMV-AR was amplified by using PCR technique. PCR product was cloned into the pGEM-T expression vector. Clones containing the vectors were selected on LB-plus ampicillin （ 100 μg · mL^-1 ） plates and sequenced. Plasmid DNA was isolated and purification after enzymatic digestion. AR fragments were initially connected with pCDNA3.1 vector. The recombinant plasmids pcDNA3.1-ARN1, pcDNA3. 1-ARN2, pcDNA3.1-ARN3, pcDNA3.1-ARC1 were extracted and confirmed by digestion with BamH 1 and Xho I enzymes. Results： Sequence analysis showed the ARN1, ARN2, ARN3, ARC1 gene fragments cloned were 100% sequence homology with that of corresponding gene coding sequence in GenBank. Conclusion： The recombinant plasmid pcDNA3.1-ARN（ 1-3 ）, pcDNA3.1-ARC1 was successfully constructed, and they could be used in vitro transcription and interpretation studies beween LRP16 and AR.