中文摘要：

Baculoviruses 生产二病毒的显型，发芽病毒(BV ) ；导出吸藏的病毒(ODV ) 。ODV 在他们开始主要感染的中肠被免除吸藏身体。由于一个离体系统的缺乏， ODV 感染的分子的机制仍然是不清楚的。这里我们表明那个 Helicoverpa 有佩带徽章权利的人的现在的数据 nucleopolyhedrovirus (HearNPV ) ODV 以一种氢离子指数依赖者方式感染了有教养的 Hz-AM1 房间。为 ODV 感染的最适 ph 是 8.5，它在中肠上皮细胞的微绒毛对那一样， ODV 本国的感染地点。抗体中立化分析显示了那四 HearNPV 口头的感染必要基因 p74， pif-1， pif-2；pif-3 为 HearNPV ODV 感染离体也是必要的。因此， HearNPV-HzAM1 系统能被用来分析 ODV 入口的机制。

英文摘要：

Baculoviruses produce two viral phenotypes, the budded virus （BV） and the occlusion-derived virus （ODV）. ODVs are released from occlusion bodies in the midgut where they initiate a primary infection. Due to the lack of an in vitro system, the molecular mechanism of ODV infection is still unclear. Here we present data demonstrating that Helicoverpa armigera nucleopolyhedrovirus （HearNPV） ODV infected cultured Hz-AM1 cells in a pH dependent manner. The optimal pH for ODV infection was 8.5, which is same to that in the microvilli of midgut epithelial cells, the ODV native infection sites. Antibodies neutralization analysis indicated that four HearNPV oral infection essential genes p74, pif-l, pif-2 and pif-3 are also essential for HearNPV ODV infection in vitro. Thus, HearNPV-HzAM1 system can be used to analyze the mechanism of ODV entry.