中文摘要：

采用原子力显微镜（AFM）研究了聚乳酸（PLLA）表面Ⅰ型胶原纳米图案化的过程。发现吸附层的形态变化与干燥条件及PLIA表面的疏水性有关。经氮气快速吹干形成的吸附层较为致密；而保持较高湿度并维持较长水膜浸润时间，由于去湿作用的影响，形成纤维网状纳米图案化结构。此结构受浸润干燥时阃、胶原浓度、基底疏水性等因素的影响，随浸润干燥时间延长，致密的胶原膜破裂，逐渐形成纳米图案化结构，胶原浓度由0．01mg／mL增加到1mg／mL均产生类似现象，且网状结构趋于紧密，PLLA分子量不同，胶原吸附层形成不同的结构形态，显示PLL～分子量对胶原的结构具有调控作用。

英文摘要：

Adsorption of type l collagen on poly（L-lactide acid）（PLLA） substrates was studied by atomic force microscopy （AFM）. The result showed that tightly collagen layers was obtained, on the one hand, by fast drying under a nitrogen flow and nano-patterned structure was obtained by slow drying under high humidity and long drying duration for dewetting occurred to the liquid-substrate interface. The discontinuous patterned structure was regulated by drying duration, concentration of collagen solution and the hydrophobieity of substrate. With increasing drying duration, continuous collagen layer ruptured and nano-pattemed structure formed ;and With increasing concentration of collagen layer, the density of the net-like structure increased. The hydrophobicity of substrates differed with the molecular weight of PLLA. The greater M^-w was, the stronger the hydrophobicity and the effect of dewetting. As a result, the adsorbed collagen structure changes and shows the regulation ability of molecular weight on it.