目的:测定不同产地、来源苦参药材中氧化苦参碱和苦参总黄酮的含量。方法:采用高效液相法检测苦参中氧化苦参碱的含量,采用紫外分光光度法检测苦参中苦参总黄酮的含量。结果:比较了10批不同产地、来源苦参药材中氧化苦参碱和苦参总黄酮的含量,其中河南产苦参(批号110613)氧化苦参碱含量最高,达19.53 mg·g-1,广西产苦参(批号110930)中苦参总黄酮含量明显高于其他产地的苦参,为14.44 mg·g-1。结论:不同产地、来源苦参药材中有效成分的含量差异较大,在临床用药时应引起重视,在含苦参的复方制剂生产中应建立药材检测的可控标准。
Objective:To study the content of oxymatrine and general flavone in Sophora flavescens from different habitats.Method:HPLC and UVspectrophotometry method were applied to detected the content of oxymatrine and general flavone in S.flavescens.Result:S.flavescens from different habitats were detected.Oxymatrin content in S.flavescens from Henan Province(Batch No:110613) was the highest,up to 19.53 mg·g-1,and general flavone content in S.flavescens from Guangxi Province(Batch No:110930) was 14.44 mg·g-1,significantly higher than the others.Conclusion:Oxymatrine and general flavone content differ widely in S.flavescens from different habitats,and it is necessary to establish quality control standard for compound preparations of S.flavescens.