中文摘要：

对紫杉醇产生菌HD1-3的孢子分别进行了紫外线照射（UV）、亚硝基胍（NTG）和uV＋NTG复合诱变处理，筛选制霉菌素抗性突变株。试验所确定的Nodulisporium sylvifoFme紫杉醇产生菌HD1-3孢子诱变的适宜条件为：将孢子悬液经过3．0mg·mL^-1。NTG、处理20min后，在电磁搅拌下，用紫外灯（30W，距离30cm）照射处理45S。共筛选出17株正突变株。经发酵筛选试验，获得了一株遗传性状稳定、高产紫杉醇的诱变菌株——UN25-3,其紫杉醇产量从出发菌株的（232．73±4．61）μg·L^-1提高至（327．14±7．63）μg·L^-1。

英文摘要：

In order to obtain resistant mutants to nystatin, ultraviolet radiation, NTG and UV ＋ NTG were used to mutagenize the spores of taxol - producing fungus HD1-3, respectively. The spore suspension was treated with 3.0 mg ·mL^- 1 NTG for 20 min, followed by UV irradiation （ 30w, 30cm distance ） for 45 s under magnetic stirring. Seventeen strains were found to be positive mutants with high taxol when screened on plate containing 95 μg· L^-1 nystatin. After further screening experiments on fermentation, a mutant -UN25-3 which was able to produce taxol with high yield and could be stably passed on genetics was eventually found, it＇ s ability to produce taxol was improved from （232.73±4. 61） μg· L^-1（strain HD1-3）to （327.14±7.63） μg· L^-1（strain UN25-3）.