中文摘要：

CCl4既作为电子受体,又是毒性极强的抑制剂,其生物毒性将影响微生物的活性、产物及生物降解的效率。采用多管发酵法（MPN）,研究了初始四氯化碳浓度对厌氧系统中两类主要微生物——发酵性细菌和硫酸盐还原菌的毒性作用,为进行地下水及土壤厌氧生物修复提供理论依据。研究表明,四氯化碳浓度越高,对发酵性细菌和硫酸盐还原菌的毒性越强;且相同浓度的CCl4对硫酸盐还原菌的抑制作用较发酵性细菌要强得多;初始浓度（V/V）0.001%～0.005%的CCl4对发酵性细菌几乎没有抑制,而对硫酸盐还原菌总数的抑制率在47%～63%以上;浓度（V/V）高于0.01%的CCl4对发酵性细菌抑制作用较强,抑制率96%～99%,对硫酸盐还原菌的抑制作用更强,抑制率高达99.9%;在相同条件下,发酵性细菌比硫酸盐还原菌对四氯化碳的耐受性要强得多,微生物对四氯化碳的耐受程度将影响四氯化碳降解效率及降解产物。

英文摘要：

Carbon tetrachloride served as electron donor and a serious inhibitor to life. The toxicity may impact the activity of microbes, the degradation rate and the biotransformation products of CCl4. Using method of most-possible-number （MPN）, this research investigated the effects of initial CCl4 concentrations on the two main microbes： fermentative bacterium （FB） and sulfate reducing bacterium （SRB） in anaerobic systems. This study may provide important evidence for anaerobic bioremediation of groundwater and soil contamination. The results indicated that increasing initial concentration of CCl4 resulted in higher toxicity to FB and SRB. The SRB was more strongly inhibited by CCl4 than FB at the same concentrations. CCl4 of 0.001%～0.005% （V/V） showed little inhibition to FB, but the inhibition to SRB was above 47%～63%. Above 0.01% （V/V） of GEl4 had very significant inhibition （96%～99%） to FB, but had much higher inhibition （99.9%） to SRB. FB was more tolerant to CCl4 than SRB under the same concentrations. The different degree of microbial tolerance to CCl4 will impact the effect of degradation and the products of transformation.